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Effects of different ectomycorrhizal fungi on somatic embryogenesis of Abies cephalonica Loud

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Abstract

The effects of ectomycorrhizal (ECM) fungi, including Laccaria bicolor (Maire) Orton, Laccaria laccata (Scop., Fr.) Berk. and Br., along with two strains of Pisolithus tinctorius (Pers.) Coker and Couch, on the proliferation and subsequent maturation of two embryogenic cell lines of Abies cephalonica Loud., designated lines 6 and 8, were investigated. In the presence of these ECM fungi, the proliferation of both embryogenic cell lines was inhibited. L. bicolor and P. tinctorius strain 2 resulted in the highest inhibition rates. On the other hand, cultivation of embryogenic cultures along with ECM fungi, termed a dual culture, increased radial growth of both P. tinctorius strains; whereas, L. bicolor and L. laccata did not grow as well in the presence of embryogenic cell masses. The dual culture during the proliferation period of embryogenic cells, however, enhanced the subsequent embryo formation and maturation of A. cephalonica; i.e. the capability of embryogenic cell lines to form somatic embryos as well as increasing the mean number of somatic embryos per 1 g fresh weight of embryogenic cell mass. However, levels of responses were highly dependent on the interaction between the specific embryogenic cell line and fungal strain.

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Abbreviations

ECM:

Ectomycorrhizal

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Acknowledgments

The research was funded by the Finnish Forest Research Institute (Metla), Academy of Finland (grant number 126488 to KN), Centre for International Mobility (CIMO) (fellowship to JK) as well as South Moravian Programme (SoMoPro, FP/2007–2013, grant no. 229603, travel grant to JK). We are grateful to Ms. Aila Viinanen and Mr. Jouko Lehto from Metla, Punkaharju Research Unit, for their technical assistance. Dr. Trevor Fenning is appreciated for his kind contribution in English revision.

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Authors and Affiliations

  1. Faculty of Forestry and Wood Technology, Mendel University, Zemědělská 3, 613 00, Brno, Czech Republic

    Jana Krajňáková

  2. Finnish Forest Industries Federation, P.O.Box 336, 00171, Helsinki, Finland

    Karoliina Niemi

  3. Faculty of Forestry, Technical University Zvolen, T. G. Masaryka 24, Zvolen, 960 53, Slovakia

    Dušan Gömöry

  4. Department of Biology, University of Oulu, P.O. Box 3000, 90014, Oulu, Finland

    Hely Häggman

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  1. Jana Krajňáková
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  2. Karoliina Niemi
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  3. Dušan Gömöry
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  4. Hely Häggman
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Correspondence to Jana Krajňáková.

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Krajňáková, J., Niemi, K., Gömöry, D. et al. Effects of different ectomycorrhizal fungi on somatic embryogenesis of Abies cephalonica Loud. Plant Cell Tiss Organ Cult 109, 353–361 (2012). https://doi.org/10.1007/s11240-011-0100-y

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