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Ectopic expression of a rice transcription factor, Mybleu, enhances tolerance of transgenic plants of Carrizo citrange to low oxygen stress

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Abstract

Oxygen deficit, which occurs in flooded or poorly drained soils, can limit plant growth and development. Low-oxygen environmental conditions also limit the distribution of many woody plants, such as citrus trees, which are considered flood-sensitive crops, although tolerance to this stress varies among genotypes and rootstocks. In this study, the rice transcription factor Mybleu was inserted into the pGA470 plant cloning vector and transferred into the epicotyl explants of the Carrizo citrange rootstock (Citrus sinensis × Poncirus trifoliata) using Agrobacterium tumefaciens-mediated transformation. The transgenic lines were confirmed for the presence and expression of the transgene, and physiological, biochemical and molecular parameters were evaluated for adaptation to hypoxic and anoxic stress conditions. The ectopic expression of Mybleu increased tolerance to oxygen deprivation in the transgenic lines, contributing to increased viability under this stress condition. This improved tolerance correlates with, and may depend on, the induction of genes and the activation of enzymes from various fermentation and carbohydrate metabolic pathways, antioxidant systems and nonsymbiotic haemoglobin-nitric oxide homeostasis mechanisms. Together, our data suggest a key role for Mybleu in coordinating the multifaceted plant response to low oxygen stress and the conservation of Mybleu-regulated pathways among species.

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Abbreviations

BA:

6-benzyladenine

CaMV:

Cauliflower mosaic virus

DTT:

Dithiothreitol

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Acknowledgments

The authors would like to thank Dr. Concetta Licciardello and Dr. Franco Faoro for scientific support and critical suggestions.

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  1. Valentina Picchi

    Present address: Unità di Ricerca per i Processi dell’Industria Agro-Alimentare, Consiglio per la Ricerca in Agricoltura, via G. Venezian 26, 20133, Milano, Italy

Authors and Affiliations

  1. Centro di Ricerca per l’Agrumicoltura e le Colture Mediterranee, Consiglio per la Ricerca in Agricoltura, Corso Savoia 190, 95024, Acireale, CT, Italy

    Paola Caruso, Donata Pietro Paolo, Giuseppe Russo & Giuseppe Reforgiato Recupero

  2. Istituto di Biologia e Biotecnologia Agraria, C.N.R., via E. Bassini 15, 20133, Milano, Italy

    Elena Baldoni, Monica Mattana, Annamaria Genga, Immacolata Coraggio, Valentina Picchi & Franca Locatelli

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Correspondence to Franca Locatelli.

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Paola Caruso and Elena Baldoni contributed equally to this work.

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Caruso, P., Baldoni, E., Mattana, M. et al. Ectopic expression of a rice transcription factor, Mybleu, enhances tolerance of transgenic plants of Carrizo citrange to low oxygen stress. Plant Cell Tiss Organ Cult 109, 327–339 (2012). https://doi.org/10.1007/s11240-011-0098-1

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