Plant Cell, Tissue and Organ Culture (PCTOC)

, Volume 106, Issue 2, pp 215–223 | Cite as

In vitro propagation of four saponin producing Maesa species

  • Ahmad Faizal
  • Ellen Lambert
  • Kenn Foubert
  • Sandra Apers
  • Danny Geelen
Original Paper

Abstract

A successful micropropagation system was developed for four different medicinal Maesa species. Multiple shoots were induced through both axillary bud formation and adventitious shoot regeneration from leaf explants. The explants were cultured on Murashige and Skoog (MS) medium supplemented with 6-benzyladenine (BA), thidiazuron (TDZ) and/or α-naphthalene acetic acid (NAA). The success of regeneration varied for different species and depended on the type and concentration of plant growth regulators. Regenerated shoots spontaneously developed roots within 6 weeks on MS hormone-free medium. The rooted shoots were transferred to the greenhouse with a 100% success rate. Furthermore, flow cytometry analysis indicated that there were no changes in ploidy level of those regenerated shoots as compared with wild type adult plants. Thin layer chromatography (TLC) analysis revealed that common and distinguishing spot of saponins were similarly observed in regenerated shoots compared to the control plants. Therefore, the protocol also provides an effective means for the in vitro conservation of Maesa spp. that produce pharmaceutically interesting saponins.

Keywords

Adventitious shoot Axillary shoot Maesa species Conservation Flow cytometry Saponin 

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Copyright information

© Springer Science+Business Media B.V. 2011

Authors and Affiliations

  • Ahmad Faizal
    • 1
  • Ellen Lambert
    • 1
  • Kenn Foubert
    • 2
  • Sandra Apers
    • 2
  • Danny Geelen
    • 1
  1. 1.Department of Plant Production, Faculty of Bioscience EngineeringGhent UniversityGhentBelgium
  2. 2.Laboratory of Pharmacognosy and Pharmaceutical Analysis, Department of Pharmaceutical Sciences, Faculty of Pharmaceutical, Biomedical and Veterinary SciencesUniversity of AntwerpAntwerpBelgium

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