In vitro propagation of four saponin producing Maesa species
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A successful micropropagation system was developed for four different medicinal Maesa species. Multiple shoots were induced through both axillary bud formation and adventitious shoot regeneration from leaf explants. The explants were cultured on Murashige and Skoog (MS) medium supplemented with 6-benzyladenine (BA), thidiazuron (TDZ) and/or α-naphthalene acetic acid (NAA). The success of regeneration varied for different species and depended on the type and concentration of plant growth regulators. Regenerated shoots spontaneously developed roots within 6 weeks on MS hormone-free medium. The rooted shoots were transferred to the greenhouse with a 100% success rate. Furthermore, flow cytometry analysis indicated that there were no changes in ploidy level of those regenerated shoots as compared with wild type adult plants. Thin layer chromatography (TLC) analysis revealed that common and distinguishing spot of saponins were similarly observed in regenerated shoots compared to the control plants. Therefore, the protocol also provides an effective means for the in vitro conservation of Maesa spp. that produce pharmaceutically interesting saponins.
KeywordsAdventitious shoot Axillary shoot Maesa species Conservation Flow cytometry Saponin
This research was funded by FWO-Flanders (project No. G.0014.08). A.F. is supported by the Directorate General of Higher Education, Ministry of National Education, Republic of Indonesia. We also acknowledge the help from Nathalie Vanden Bleeken for improving the saponin detection protocol.
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