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Biochemical and molecular profiling of micropropagated and conventionally grown Kaempferia galanga

  • Sujata Mohanty
  • Reena Parida
  • Sikha Singh
  • Raj Kumar Joshi
  • Enketeswara Subudhi
  • Sanghamitra NayakEmail author
Original Paper

Abstract

Kaempferia galanga L., family Zingiberaceae, is used extensively in the preparation of both traditional and modern medicines. Buds of rhizomes of K. galanga were incubated on Murashige and Skoog (MS) medium supplemented with 1 mg/l benzyladenine and 0.5 mg/l indole-3-acetic acid (IAA) to induce shoot proliferation. Micropropagated plantlets subjected to random amplified polymorphic DNA (RAPD) and inter simple sequence repeat (ISSR) marker-based molecular profiling revealed uniform banding patterns similar to those of the mother plants. After 2 years of culture in vitro, plantlets were transplanted to the field, evaluated for different agronomic traits, and their rhizomes were subjected to biochemical profiling using quantitative and qualitative assays of essential oils. Gas chromatography and mass spectroscopy analysis of rhizome oil of micropropagated plants showed the presence of 10 major components which were similar to those detected in the mother plants, and accounted for 95.5% of the total compounds. The compound ethyl-p-methoxy cinnamate accounted for 59.5% of the total compounds detected, followed by ethyl cinnamate, 3-carene, pentadecane, borneol, bornyl acetate, delta-selinene, camphor, alpha-piene and immidazole, 5-carbonylvinyl-4-nitro. Biochemical and molecular profiling of micropropagated clones revealed that an in vitro micropropagation protocol could be effectively used for commercial propagation of true-to-type K. galanga for a stable supply of the medicinal compounds present in this plant species.

Keywords

Genetic stability Essential oil Kaempferia galanga Micropropagation Molecular marker 

Notes

Acknowledgments

The authors are grateful to Dr. Sudam Chandra Si, Dean and Dr. Manoj Ranjan Nayak, President, Center of Biotechnology, Siksha O Anusandhan University, for providing all facilities.

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Copyright information

© Springer Science+Business Media B.V. 2010

Authors and Affiliations

  • Sujata Mohanty
    • 1
  • Reena Parida
    • 1
  • Sikha Singh
    • 1
  • Raj Kumar Joshi
    • 1
  • Enketeswara Subudhi
    • 1
  • Sanghamitra Nayak
    • 1
    Email author
  1. 1.Centre of BiotechnologySiksha O Anusandhan UniversityBhubaneswarIndia

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