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Changes in DNA-methylation during zygotic embryogenesis in interspecific hybrids of beans (Phaseolus ssp.)

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Abstract

Hybrid embryos resulting from crosses between Phaseolus species often fail to reach maturity and some combinations frequently abort at early developmental stages. The genetic or molecular basis for these consistent developmental defects is at present not clear. However, an extremely complex genetic system, thought to be caused by major epigenetic changes associated with gene expression changes, has been shown to be active in plant species. We have investigated DNA methylation in two interspecific hybrids, Phaseolus vulgaris × Phaseolus coccineus and its reciprocal crosses, using methylation sensitive amplification polymorphism (MSAP). The potential use of MSAP for detecting methylation variation during embryogenesis in interspecific hybrids is discussed. Significant differences in the DNA methylation patterns were observed in abortive (interspecific hybrids) and non abortive (parental) genotypes. Taken together, our results strongly suggest that generalized alterations in DNA methylation profiles could play a causative role in early interspecific embryo abortion in vivo. A considerable change in the methylation pattern during embryogenesis could be involved in the disruption of the regulation or maintenance of the embryogenesis process of Phaseolus interspecific hybrids. The results also support the earlier hypothesis that DNA methylation is critical for the regulation of plant embryogenesis and gene expression.

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Abbreviations

DAP:

Days after pollination

MSAP:

Methylation sensitive amplification polymorphism

RT–PCR:

Reverse transcription polymerase chain reaction

SSR:

Simple sequence repeats

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Authors and Affiliations

  1. University of Liège-Gembloux Agro-Bio Tech Unit of Tropical Crop Husbandry and Horticulture, Gembloux Agricultural University, Passage des Déportés 2, 5030, Gembloux, Belgium

    Ghassen Abid, André Toussaint & Jean-Pierre Baudoin

  2. Department of Life Sciences, Unit of Breeding and Biodiversity, Walloon Agricultural Research Centre, Chaussée de Charleroi, 234, 5030, Gembloux, Belgium

    Ghassen Abid, Yordan Muhoviski, Jean-Marie Jacquemin & Dominique Mingeot

  3. Department of Agronomy and Plant Biotechnology, Laboratory of Agronomy, Avenue Charles Nicolle, 43, 1082, Tunis, Tunisia

    Khaled Sassi

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  1. Ghassen Abid
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  2. Yordan Muhoviski
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  3. Jean-Marie Jacquemin
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  4. Dominique Mingeot
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  5. Khaled Sassi
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  6. André Toussaint
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  7. Jean-Pierre Baudoin
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Correspondence to Ghassen Abid.

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Abid, G., Muhoviski, Y., Jacquemin, JM. et al. Changes in DNA-methylation during zygotic embryogenesis in interspecific hybrids of beans (Phaseolus ssp.). Plant Cell Tiss Organ Cult 105, 383–393 (2011). https://doi.org/10.1007/s11240-010-9878-2

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  • DOI: https://doi.org/10.1007/s11240-010-9878-2

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