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Transgenic tomato plants expressing a wheat endochitinase gene demonstrate enhanced resistance to Fusarium oxysporum f. sp. lycopersici

  • P. V. Girhepuje
  • G. B. ShindeEmail author
Original Paper

Abstract

Various chitinases have been shown to inhibit the growth of fungal pathogens in in vitro as well as in planta conditions. chi194, a wheat chitinases gene encoding a 33-kDa chitinase protein, was overexpressed in tomato plants (cv. Pusa Ruby) under the control of maize ubiquitin 1 promoter. The integration of transgene in tomato plants was confirmed with polymerase chain reaction (PCR) and Southern blot analysis. The inheritance of the transgene in T1 and T2 generations were shown by molecular analysis and the hygromycin sensitivity test. The broad range of chitinase activity was observed among the transgenic lines in T0 and a similar range was retained in the T1 and T2 generations. Most importantly, the transgenic tomato lines with high chitinase activity were found to be highly resistant to the fungal pathogen Fusarium oxysporum f. sp. lycopersici. Thus, the results demonstrated that the expression of the wheat endochitinase chi194 in tomato plants confers resistance against Fusarium wilt disease caused by the fungal pathogen Fusarium oxysporum f. sp. lycopersici.

Keywords

Fusarium oxysporum Endochitinase Disease resistance Transgenic tomato 

Notes

Acknowledgments

We thank Prof. N. Sakthivel, Department of Biotechnology, Pondicherry University, India, and Dr. S. Muthukrishnan, Department of Biochemistry, Kansas State University, USA, for kindly providing the plasmids for this study.

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Copyright information

© Springer Science+Business Media B.V. 2010

Authors and Affiliations

  1. 1.Post-Graduate Teaching Department of BiochemistryRTM Nagpur UniversityNagpurIndia

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