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PMI (manA) as a nonantibiotic selectable marker gene in plant biotechnology

  • P. StoykovaEmail author
  • P. Stoeva-Popova
Review

Abstract

Due to the rising public concern over the use of genes conferring antibiotic and herbicide resistance, alternative systems for selection after plant transformation are being developed. A positive selection system consists of a physiologically inert metabolite as the selection agent and a respective gene which determines a metabolic advantage via selection agent utilization. The transformed cells are able to overcome the suppressive effects of the selection, while the untransformed ones starve but are not killed. The enzyme phosphomannose isomerase (PMI, E.C. 5.3.1.8) catalyzes the reversible interconversion of mannose-6-phosphate and fructose-6-phosphate in prokaryotic and eukaryotic organisms. The PMI selection system is called positive due to the effect of “starvation” caused to the nontransformed plant tissue because of its incapability to utilize mannose as a carbon source. In this mini-review we researched the literature to obtain a more detailed view of the characteristics, specifics, problems, and advantages of applying the PMI/mannose selection system.

Keywords

Mannose Phosphomannose isomerase Positive selection Plant genetic transformation 

Abbreviations

Man

Mannose

M-6-P

Mannose-6-phosphate

PMI

Phosphomannose isomerase

TE

Transformation efficiency

ATP

Adenosine triphosphate

PCR

Polymerase chain reaction

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Copyright information

© Springer Science+Business Media B.V. 2010

Authors and Affiliations

  1. 1.AgroBioInstituteSofiaBulgaria
  2. 2.Department of BiologyWinthrop UniversityRock HillUSA

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