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Shoot organogenesis and somatic embryogenesis from leaf and shoot explants of Ochna integerrima (Lour)

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Abstract

Ochna integerrima is a medicinal and ornamental plant in Southeastern Asia. It has been listed as a rare and endangered species in China. Here we studied the effects of plant growth regulators and their concentrations on the induction of somatic embryogenesis and shoot organogenesis from leaf and shoot explants of O. integerrima for the first time. Cytokinins played a crucial role in somatic embryogenesis and shoot organogenesis. Among them, a higher concentration of thidiazuron (10.0–15.0 μM TDZ) could induce both somatic embryogenesis and adventitious shoot formation whereas low concentrations of TDZ (5.0 μM) could only induce adventitious shoots. However, 6-benzyladenine (BA at 5–15 μM) could only induce adventitious shoots. Shoot explants induced more adventitious shoots and somatic embryos than leaf explants when cultured on medium with the same concentration (5–15 μM) of TDZ or 15 μM BA. Medium containing 0.5 μM α-naphthaleneacetic acid and 8 μM indole-3-butyric acid and 0.1% activated charcoal could induce adventitious roots within 1 month. An efficient mass propagation and regeneration system has been established.

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Abbreviations

2,4-D:

2,4-dichloro-phenoxyacetic acid

BA:

6-benzyladenine

IBA:

Indole-3-butyric acid

NAA:

α-naphthaleneacetic acid

TDZ:

Thidiazuron

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Authors and Affiliations

  1. Key Laboratory of Plant Resources Conservation and Sustainable Utilization, South China Botanical Garden, The Chinese Academy of Sciences, 510650, Guangzhou, China

    Guohua Ma, Jinfeng Lü, Xinhua Zhang & Jietang Zhao

  2. Graduate University of Chinese Academy of Sciences, 100039, Beijing, China

    Jinfeng Lü

  3. Faculty of Agriculture and Graduate School of Agriculture, Kagawa University, Miki-cho Ikenobe 2393, Kagawa-ken, 761-0795, Japan

    Jaime A. Teixeira da Silva

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  1. Guohua Ma
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  2. Jinfeng Lü
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  3. Jaime A. Teixeira da Silva
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  4. Xinhua Zhang
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  5. Jietang Zhao
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Correspondence to Guohua Ma.

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Ma, G., Lü, J., da Silva, J.A.T. et al. Shoot organogenesis and somatic embryogenesis from leaf and shoot explants of Ochna integerrima (Lour). Plant Cell Tiss Organ Cult 104, 157–162 (2011). https://doi.org/10.1007/s11240-010-9812-7

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  • DOI: https://doi.org/10.1007/s11240-010-9812-7

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