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Teratomas of Drosera capensis var. alba as a source of naphthoquinone: ramentaceone

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Abstract

Plants belonging to genus Drosera (family Droseraceae) contain pharmacologically active naphthoquinones such as ramentaceone and plumbagin. Hairy root cultures obtained following Agrobacterium rhizogenes-mediated transformation have been reported to produce elevated levels of secondary compounds as well as exhibit desirable rapid biomass accumulation in comparison to untransformed plants. The aim of this study was to establish hairy root or teratoma cultures of Drosera capensis var. alba and to increase the level of ramentaceone in transformed tissue by application of abiotic and biotic elicitors. The appearance of transformed tissues—teratomas but not hairy roots was observed 18 weeks after transformation. The transformation efficiency was 10% and all teratoma cultures displayed about 3 times higher growth rate than non-transformed cultures of D. capesis. The transformation was confirmed by PCR and Southern hybridization using primers based on the A. rhizogenes rolB and rolC gene sequences. HPLC analysis of ramentaceone content indicated 60% higher level of this metabolite in teratoma tissue in comparison to non-transformed cultures. Among the elicitors tested jasmonic acid (2.5 mg l−1) turned out to be the most effective. The productivity of ramentaceone in elicited teratoma cultures was about sevenfold higher than in liquid cultures of D. capensis var. alba and amounted to 2.264 and 0.321 mg respectively during 4 weeks of cultivation. This is the first report on the transformation of Drosera plant with A. rhizogenes.

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Abbreviations

CTAB:

Hexadecyltrimethylammoniumbromide

DW:

Dry weight

FW:

Fresh weight

JA:

Jasmonic acid

MBC:

Minimal Bactericidal Concentration

MIC:

Minimal Inhibitory Concentration

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Acknowledgments

This work was supported by DS/0051-4-0010-9 and the Foundation for Polish Science grant START for Anna Szpitter.

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Authors and Affiliations

  1. Department of Biotechnology, Laboratory of Plant Protection and Biotechnology, Intercollegiate Faculty of Biotechnology, University of Gdansk and Medical University of Gdansk, Kladki 24, 80-822, Gdansk, Poland

    Aleksandra Krolicka, Anna Szpitter, Anna Gwizdek-Wisniewska & Ewa Lojkowska

  2. Department of Genetics, Plant Breeding and Seed Science, University of Agriculture in Krakow, Al. 29 Listopada 54, 31-425, Krakow, Poland

    Krzysztof Stawujak & Rafal Baranski

  3. Department of Analytical Chemistry, Gdansk University of Technology, Gabriela Narutowicza 11/12, 80-952, Gdansk, Poland

    Anita Skrzypczak & Marian Kaminski

Authors
  1. Aleksandra Krolicka
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  2. Anna Szpitter
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  3. Krzysztof Stawujak
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  4. Rafal Baranski
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  5. Anna Gwizdek-Wisniewska
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  6. Anita Skrzypczak
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  7. Marian Kaminski
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  8. Ewa Lojkowska
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Correspondence to Aleksandra Krolicka.

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Krolicka, A., Szpitter, A., Stawujak, K. et al. Teratomas of Drosera capensis var. alba as a source of naphthoquinone: ramentaceone. Plant Cell Tiss Organ Cult 103, 285–292 (2010). https://doi.org/10.1007/s11240-010-9778-5

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  • DOI: https://doi.org/10.1007/s11240-010-9778-5

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