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Cloning and characterization of a potato TFL1 gene involved in tuberization regulation

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Abstract

Both tuberization and flowering are influenced by photoperiod in potato. TERMINAL FLOWER1 is a key regulator of floral timing in Arabidopsis and other herbaceous species. In order to clarify the relationship of TFL1 protein with tuberization, a homolog of TFL1, designated Solanum tuberosum TFL1 (StTFL1; accession no. DQ307621), was isolated from potato plantlets by reverse transcriptase-PCR and rapid amplification of cDNA ends. The predicted amino acid sequence of this cDNA had a high degree of identity with other homologous members of the phosphatidylethanolamine-binding protein family. Analysis of mRNA levels for StTFL1 showed that it was highly expressed in roots and initial stolons, with the expression becoming progressively weaker during subsequent tuberization. Over-expression of StTFL1 resulted in normal tuberization in transgenic lines under the long-day condition. These results demonstrate that StTFL1 is involved in the regulation of tuberization.

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Abbreviations

CEN:

CENTRORADIALIS

PEBP:

Phosphatidylethanolamine-binding protein

TFL1:

TERMINAL FLOWER1

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Acknowledgments

This study was financially supported by grants from the opening foundation of the Jiangsu Key Laboratory for Eco-Agricultural Biotechnology around Hongze Lake (NO. HZHL0807) and the State Key Laboratory of Crop Genetics and Germplasm Enhancement, The Ministry of Sciences and Technology of the People’s Republic of China (NO. ZW2007003).

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Authors and Affiliations

  1. College of Life Sciences, Nanjing Agricultural University, 210095, Nanjing, China

    Jian-Lin Guo, Chun-Lin Yu, Chun-Yuan Fan, Qi-Neng Lu, Jing-Ming Yin, Yun-Feng Zhang & Qing Yang

  2. Institute of Botany, Jiangsu Province and Chinese Academy of Sciences, Jiangsu Province Key Laboratory for Plant Ex Situ Conservation, 210014, Nanjing, China

    Jian-Lin Guo

  3. Jiangsu Key Laboratory for Eco-Agricultural Biotechnology around Hongze Lake, 223300, Huai’an city, China

    Yun-Feng Zhang

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  1. Jian-Lin Guo
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  2. Chun-Lin Yu
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  7. Qing Yang
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Correspondence to Qing Yang.

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Guo, JL., Yu, CL., Fan, CY. et al. Cloning and characterization of a potato TFL1 gene involved in tuberization regulation. Plant Cell Tiss Organ Cult 103, 103–109 (2010). https://doi.org/10.1007/s11240-010-9759-8

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  • DOI: https://doi.org/10.1007/s11240-010-9759-8

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