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Adventitious shoot regeneration from leaf explants of southern highbush blueberry cultivars

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Abstract

Protocols were developed to optimize adventitious shoot regeneration from four southern highbush blueberry cultivars. Leaf explants from 6 week-old shoots of the four cultivars were excised and cultured on woody plant medium each containing thidiazuron (4.54 or 9.08 μM), zeatin (18.2 μM), or zeatin riboside (5.7 or 11.4 μM) either separately or in combination with α-naphthaleneacetic acid at 2.69 μM. Optimum medium for shoot regeneration was genotype-dependent. Efficient regeneration was obtained at frequencies of 88.9% for ‘Jewel’, 87.8% for ‘Emerald’, 53.3% for ‘Jubilee’ and 87.8% for ‘Biloxi’. Leaf explants of newly developed shoots from the cultures having undergone five subcultures had higher regeneration frequencies than those having undergone two subcultures. Regenerated shoots, 80–100% for each cultivar, rooted in 8 weeks after transplantation to soil. The regeneration systems described have potential use in genetic transformation of southern highbush blueberry cultivars.

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Fig. 1

Abbreviations

MS:

Murashige and Skoog

NAA:

α-naphthaleneacetic acid

TDZ:

Thidiazuron

WPM:

Woody plant medium

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Acknowledgments

This research was supported in part by MSU Project GREEEN (Generating Research and Extension to Meet Economic and Environmental Needs) and in part by a specific cooperative agreement between the USDA/ARS and MSU.

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Author notes

  1. Cheng Liu

    Present address: Liaoning Institue of Pomology, 115009, Xiongyue, Liaoning, People’s Republic of China

Authors and Affiliations

  1. Plant Biotechnology Resource and Outreach Center, Department of Horticulture, Michigan State University, East Lansing, MI, 48824, USA

    Cheng Liu, Pete Callow, James F. Hancock & Guo-qing Song

  2. USDA-ARS, Genetic Improvement of Fruits and Vegetables Lab, Beltsville, MD,  20705, USA

    Lisa J. Rowland

Authors
  1. Cheng Liu
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  2. Pete Callow
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  3. Lisa J. Rowland
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  4. James F. Hancock
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  5. Guo-qing Song
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Corresponding author

Correspondence to Guo-qing Song.

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Liu, C., Callow, P., Rowland, L.J. et al. Adventitious shoot regeneration from leaf explants of southern highbush blueberry cultivars. Plant Cell Tiss Organ Cult 103, 137–144 (2010). https://doi.org/10.1007/s11240-010-9755-z

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  • DOI: https://doi.org/10.1007/s11240-010-9755-z

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