Plant Cell, Tissue and Organ Culture (PCTOC)

, Volume 103, Issue 1, pp 23–31 | Cite as

In vitro regeneration in Dierama erectum Hilliard

Original Paper

Abstract

The genus Dierama comprises plants with a potential to be developed as ornamentals. D. erectum seeds were decontaminated and germinated on 1/10th strength Murashige and Skoog (Physiol Plant 15:473–497, 1962) (MS) media without plant growth regulators or sucrose. In an experiment investigating the effects of 6-benzyladenine (BA), meta-Topolin (mT), kinetin (KIN) and zeatin (Z) with or without α-naphthaleneacetic acid (NAA), the highest shoot number per hypocotyl (4.20 ± 0.51) was obtained from MS medium supplemented with 1.0 μM Z after 8 weeks. This was followed by a combination of 2.0 μM KIN and 2.0 μM NAA with 3.67 ± 0.81 shoots per explant. BA treatments produced 3.20 ± 0.22 shoots per hypocotyl explant when 2.0 μM was combined with 1.0 μM NAA, while mT gave 3.09 ± 0.99 shoots per explant when 2.0 μM mT was combined with 2.0 μM NAA. Adventitious shoot regeneration was optimised when shoots were grown under a 16-h photoperiod at 100 μmol m−2 s−1 on MS medium supplemented with 1.0 μM BA. This resulted in an average of 12.73 ± 1.03 shoots per hypocotyl explant. Various concentrations of ancymidol, activated charcoal and sucrose did not promote in vitro corm formation of this species. Plants rooted successfully after 8 weeks on MS medium supplemented with 1.0 μM indole-3-butyric acid (IBA) and had an average root number of 2.73 ± 0.40. After 2 months of acclimatisation, plants had formed corms. The largest corms (of diameter 0.45 ± 0.03 cm) were produced in plants pre-treated with 0.5 μM IBA. The highest plant survival percentage of 73% was also associated with this treatment.

Keywords

Cytokinins Dierama erectum Micropropagation Photoperiod Threatened species 

Abbreviations

ANOVA

Analysis of variance

BA

6-benzyladenine

DMRT

Duncan’s multiple range test

HCl

Hydrochloric acid

IAA

Indole-3-acetic acid

IBA

Indole-3-butyric acid

KIN

Kinetin

MS

Murashige and Skoog medium

mT

meta-Topolin

NAA

α-naphthaleneacetic acid

PGR

Plant growth regulator

KOH

Potassium hydroxide

NaOCl

Sodium hypochlorite

PPFD

Photosynthetic photon flux density

Z

Zeatin

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Copyright information

© Springer Science+Business Media B.V. 2010

Authors and Affiliations

  1. 1.Research Centre for Plant Growth and Development, School of Biological and Conservation SciencesUniversity of KwaZulu-Natal PietermaritzburgScottsvilleSouth Africa

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