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Effect of salinity on antioxidant enzymes in calli of the halophyte Nitraria tangutorum Bobr.

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Abstract

Nitraria tangutorum Bobr., a typical desert halophyte, plays an important ecological role because of its superior tolerance to severe drought and high salinity. Very little is known about the physiological adaptative mechanism of this species to environmental stresses. The aim of this study was to investigate the changes of antioxidant enzyme activities and the regulatory mechanism of ascorbate peroxidase (APX) activity in the calli from Nitraria tangutorum Bobr. after treatment with different NaCl concentrations. The activities of superoxide dismutase (SOD) and catalase (CAT) significantly increased in the calli treated with NaCl, while the peroxidase activity decreased. APX activity was also elevated significantly in response to NaCl, but the increase was partly abolished by H2O2 scavenger dimethylthiourea (DMTU). Furthermore, the excitatory effect of salinity on APX could be alleviated by the addition of exogenous CAT and nicotinamide adenine dinucleotide phosphate (NADPH) oxidase inhibitor diphenylene iodonium, indicating that the modulation of the APX activity in Nitraria tangutorum Bobr. calli might be associated with NADPH oxidase-dependent H2O2 generation. Measurement and analysis using fluorescent dye 2′,7′-dichlorodihydrofluorescein diacetate showed the increase of H2O2 content in salinity-treated calli. The investigation of NADPH-dependent O −2 production in plasma membrane (PM) vesicles isolated from Nitraria tangutorum Bobr. calli revealed that salinity treatment stimulated NADPH oxidase activity. In conclusion, these results suggest that the higher activities of antioxidant enzymes play an important role in the salt tolerance of Nitraria tangutorum Bobr. calli and that the extracellular production of H2O2, depending on the excitation of PM NADPH oxidase, is responsible for enhancing the APX activity in Nitraria tangutorum Bobr. calli under salinity stress.

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Abbreviations

APX:

Ascorbate peroxidase

ASA:

Ascorbate

CAT:

Catalase

DMTU:

Dimethylthiourea

DPI:

Diphenylene iodonium

DTT:

1,4-dithiothreitol

EDTA:

Ethylenediaminetetraacetic acid

FW:

Fresh weight

H2DCF-DA:

2′,7′-dichlorodihydrofluorescein diacetate

H2O2 :

Hydrogen peroxide

LSCM:

Laser scanning confocal microscope

MS:

Murashige and Skoog

NADPH:

Nicotinamide adenine dinucleotide phosphate

NBT:

Nitroblue tetrazolium

PBS:

Phosphate-buffered saline

PM:

Plasma membrane

PMSF:

Phenylmethanesulfonyl fluoride

POD:

Peroxidase

PVP:

Polyvinylpyrrolidone

ROS:

Reactive oxygen species

SOD:

Superoxide dismutase

Tris:

Tris-(hydroxymethyl) amino methane

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Acknowledgements

This work is financially supported by the National Natural Science Foundation of China (grant no. 30960064) and the National Science Foundation for Distinguished Young Scholars of China (grant no. 30625008).

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Authors and Affiliations

  1. School of Life Science, Northwest Normal University, Lanzhou, Gansu, 730070, People’s Republic of China

    Yingli Yang, Ruxia Shi, Xueling Wei & Qing Fan

  2. State Key Laboratory of Arid Agroecology, School of Life Science, Lanzhou University, Lanzhou, 730000, People’s Republic of China

    Lizhe An

  3. Cold and Arid Regions Environmental and Engineering Research Institute, Chinese Academy of Sciences, Lanzhou, 730000, People’s Republic of China

    Yingli Yang & Lizhe An

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  1. Yingli Yang
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  2. Ruxia Shi
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  3. Xueling Wei
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  4. Qing Fan
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Correspondence to Yingli Yang.

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Yang, Y., Shi, R., Wei, X. et al. Effect of salinity on antioxidant enzymes in calli of the halophyte Nitraria tangutorum Bobr.. Plant Cell Tiss Organ Cult 102, 387–395 (2010). https://doi.org/10.1007/s11240-010-9745-1

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  • DOI: https://doi.org/10.1007/s11240-010-9745-1

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