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Eradication of endophytic bacteria via treatment for axillary buds of Petunia hybrida using Plant Preservative Mixture (PPMTM)

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Abstract

Plant Preservative Mixture (PPMTM), a proprietary mixture of two broad-spectrum isothiazolone biocides, was recently introduced as a prophylactic anti-bacterial agent in plant tissue culture. Its efficacy for eradicating endophytic Sphingomonas paucimobilis in Petunia hybrida was tested. The presence of bacterial cells in intercellular spaces of parenchyma tissues in the cortex and pith, in the lumen of xylem vessels and within sclerenchyma cells, could be detected in histo-sections by both light and transmission electron microscopy. The eradication protocol involved infiltration of infected axillary buds with PPM solution under vacuum. Quantitation by high performance liquid chromatography (HPLC) revealed that axillary buds from upper young nodes absorbed significantly more PPM compared with those from the lower nodes. Indexation indicated successful eradication of bacterial contaminants from upper young axillary buds after vacuum-infiltration with 5 ml l−1 PPM.

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Abbreviations

CMI:

Chloromethylisothiazolone

HPLC:

High performance liquid chromatography

MI:

Methylisothiazolone

PPM:

Plant Preservative Mixture

TEM:

Transmission electron microscopy

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Authors and Affiliations

  1. Department of Environmental & Aquatic Sciences, Curtin University of Technology, GPO Box U1987, Perth, WA, 6845, Australia

    Junji Miyazaki & Beng H. Tan

  2. Department of Applied Chemistry, Curtin University of Technology, GPO Box U1987, Perth, WA, 6845, Australia

    Steve G. Errington

  3. CSIRO Plant Industry, Myall Vale, Locked Bag 59, Narrabri, NSW, 2390, Australia

    Junji Miyazaki

Authors
  1. Junji Miyazaki
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  2. Beng H. Tan
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  3. Steve G. Errington
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Correspondence to Junji Miyazaki.

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Miyazaki, J., Tan, B.H. & Errington, S.G. Eradication of endophytic bacteria via treatment for axillary buds of Petunia hybrida using Plant Preservative Mixture (PPMTM). Plant Cell Tiss Organ Cult 102, 365–372 (2010). https://doi.org/10.1007/s11240-010-9741-5

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  • DOI: https://doi.org/10.1007/s11240-010-9741-5

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