Skip to main content
SpringerLink
Log in

The dual role of carbenicillin in shoot regeneration and somatic embryogenesis of horseradish (Armoracia rusticana) in vitro

  • Research Note
  • Published:
Plant Cell, Tissue and Organ Culture (PCTOC) Aims and scope Submit manuscript

Abstract

Carbenicillin, a well-known antibiotic, has been reported to have growth regulator-like activity in vitro for some plant species. In the present paper we add horseradish (Armoracia rusticana) to the list of plants exhibiting such responses. This project began as an effort to eliminate latent bacterial contamination among established in vitro horseradish plants. Carbenicillin (100 mg L−1) added to regeneration medium eliminated all visible bacterial contaminants. Unexpectedly, carbenicillin-grown explants regenerated adventitious shoots faster (14 days) than those on control medium (21 days). In addition eight of 11 horseradish cultivars grown on carbenicillin produced more adventitious shoots per explant than control. At much higher levels (2,000 mg L−1) carbenicillin was found to retard somatic embryogenic callus induction. Based on these observations we suggest that carbenicillin at moderate levels enhances shoot development in horseradish. The mode of action of carbenicillin’s growth regulator-like activity needs to be investigated.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3

References

  • Barrett C, Cassells AC (1994) An evaluation of antibiotics for the elimination of Xanthomonas campestris pv pelargonii (brown) from Pelargonium × domesticum cv grand slam explants in vitro. Plant Cell Tiss Organ Cult 36(2):169–175

    Article  CAS  Google Scholar 

  • Bhau BS, Wakhlu AK (2001) Effect of some antibiotic on the in vitro morphogenetic response from callus cultures of Coryphantha elephantidens. Biologica Plantarum 44(1):19–24

    Article  CAS  Google Scholar 

  • Blake J (1988) Mites and thrips as bacterial and fungal vectors between plant tissue cultures. Acta Hortic 225:163–166

    Google Scholar 

  • Dai W, Castillo C (2007) Factors affecting plant regeneration from leaf tissues of Buddleia species. HortScience 42:1509–1517

    Google Scholar 

  • de Andrade SRM, Faleiro FG, Teixeira JB, de Pinto ACQ, Radel G, Cordeiro MCR, Ramo VHV (2009) Uses of alternative antibiotics to control bacterium growth in mango explants. Acta Hortic 820:541–546

    Google Scholar 

  • Estopa M, Marfa V, Mele E, Messeguer J (2001) Study of different antibiotic combinations for use in the elimination of Agrobacterium with kanamycin selection in carnation. Plant Cell Tiss Organ Cult 65(3):211–220

    Article  CAS  Google Scholar 

  • Holford P, Newbury HJ (1992) The effects of antibiotics and their breakdown products on the in vitro growth of Antirrihinum majus. Plant Cell Rep 11(2):93–96

    Article  CAS  Google Scholar 

  • Houwe IVD, Guns J, Swennen R (1998) Bacterial contamination in Musa shoot tip cultures. Proceedings of the first international symposium on banana in the subtropics, Puerto De La Cruz, Tenerife, Spain, pp. 485–492

  • Klopmeyer MJ (2000) Indexing for plant pathogens. Plant tissue culture concepts and laboratory exercises, 2nd edn. CRC Press, USA, pp 417–425

    Google Scholar 

  • Knauss JF, Miller JW (1978) Contaminant, Erwinta carotovora, affecting commercial plant tissue culture. In vitro J Tiss Cult Assoc 14(9):754–756

    Google Scholar 

  • Lapidus VL, Donskaya NG, Libinson GS, Tochenaya NP (1976) Stability in aqueous solutions and acidic properties of carbenicillin. Khimiko-Farmatsevticheskii Zhurnal 10(6):112–114

    CAS  Google Scholar 

  • Leifert C, Cassels AC (2001) Microbial hazards in plant tissue and cell cultures. In vitro Cell Dev Biol-Plant 37(2):122–138

    Google Scholar 

  • Leifert C, and Woodward S (1998). Laboratory contamination management: the requirement for microbiological quality assurance. Second International Symposium on Bacterial and Bacteria-Like Contaminants of Plant Tiss Cult 52: 83–88

  • Lin JJ, Assad-Garcia N, Kuo J (1995) Plant hormone effect of antibiotics on the transformation efficiency of plant tissues by Agrobacterium tumefaciens cells. Plant Sci (Limerick) 109(2):171–177

    Article  CAS  Google Scholar 

  • Liu F, Cao MQ, Sato T (1993) Effects of several antibiotics on the morphogenesis of explants culture in vitro. Acta Agriculturae Boreali-Sinica 8(1):47–51

    Google Scholar 

  • Luna C, Collavino M, Mroginski L, Sansberro P (2008) Identification and control of bacterial contaminants from ilex dumosa nodal segments culture in a temporal immersion bioreactor system using 16S rDNA analysis. Plant Cell. Tiss Organ Cult 95(1):13–19

    Article  CAS  Google Scholar 

  • Manual Duchefa (1990) Antibiotics, mode of action, spectrum, solubility, sterilisation, stability. Duchefa Pharmaceuticals and biochemicals technical manual. Haarlem, The Netherlands

    Google Scholar 

  • Meyer MM Jr, Milbrath GM (1977) In vitro propagation of horseradish with leaf pieces. HortScience 12(6):544–545

    Google Scholar 

  • Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue culture. Physiol Plant 15:473–497

    Article  CAS  Google Scholar 

  • Nakano M, Mii M (1993) Antibiotics stimulate somatic embryogenesis without plant growth regulators in several Dianthus cultivars. J Plant Physiol 141:721–725

    CAS  Google Scholar 

  • Nauerby B, Billing K, Wyndaele R (1997) Influence of the antibiotic timentin on plant regeneration compared to carbenicillin and cefotaxime in concentrations suitable for elimination of Agrobacterium tumefaciens. Plant Sci (Limerick) 123(1/2):169–177

    Article  CAS  Google Scholar 

  • Norton M, Uchanski M, Scoggins K, Skirvin RM (2001) Tissue culture project progress. Horseradish Research Review and Proceedings from the Horseradish Growers School. University of Illinois Ext. pp. 18–20

  • Pollock K, Barfield DG, Shield R (1983) The toxicity of antibiotics to plant cell cultures. Plant Cell Rep 2:36–39

    CAS  Google Scholar 

  • Rahman LU, Ikenaga T, Kitamura Y (2004) Penicillin derivatives induce chemical structure-dependent root development, and application for plant transformation. Plant Cell Rep 22(9):668–677

    Article  Google Scholar 

  • Reed BM, Buckley PM, Dewilde TN (1995) Detection and eradication of endophytic bacteria from micropropagated mint plants. In Vitro Cell Dev Biol 31:53–57

    Article  Google Scholar 

  • SAS Institute (1999) Reference Manual for version 8. SAS Institute Inc. Cary, NC 27513 USA

  • Shehata AM (2004) Developing a system to produce uniform micropropagated horseradish (Armoracia rusticana) plants in vitro. Ph.D. Thesis. University of Illinois at Urbana-Champaign, USA

  • Suzuki S, Nakano M (2003) Effects of antibiotics and bialaphos on the growth and development of embryogenic callus cultures of Muscari armeniacum. Biologia Plantarum 47(3):425–427

    Article  Google Scholar 

  • Suzuki S, Niimi Y, Sakakibara T, Hosokawa K, Yamamura S, Nanko M (1989) Effects of several antibiotics and bialaphos on the growth and organ formation of Lilium formosanum calli. Plant Biotechnol 15:213–216

    Google Scholar 

  • Suzuki S, Oota M, Nakano M (2002) Embryogenic callus induction from leaf explants of the Liliaceous ornamental plant, Agapanthus praecox ssp. orientalis (Leighton), Leighton Histological study and response to selective agents. Sci Hortic 95(1/2):123–132

    Article  CAS  Google Scholar 

  • Tang W, Harris L, Newton RJ (2003) Influences of antibiotics on plantlet regeneration via organogenesis in loblolly pine (Pinus taeda L.). J For Res 14(3):185–190

    Article  CAS  Google Scholar 

  • Tanprasert P, Reed BM (1997) Determination of minimal bactericidal and effective antibiotic treatment concentrations for bacterial contaminants from micropropagated strawberries. In vitro Cell Dev Biol—Plant 33(3):227–230

    Article  CAS  Google Scholar 

  • Yu TA, Yeh SD, Yang JS (2001) Effects of carbenicillin and cefotaxime on callus growth and somatic embryogenesis from adventitious roots of papaya. Bot Bull Acad Sin 42:281–286

    CAS  Google Scholar 

  • Uchanski ME (2007) Yield and quality of pathogen-free horseradish (Armoracia rusticana) planting stock. Ph.D. Thesis. University of Illinois at Urbana-Champaign. USA

  • Xu ChenXi, Jing J, Jun Tao L (2007) Effects of several antibiotics on adventitious bud regeneration from in vitro leaves of Populus davidiana Dode x P. Lauche. J Northeast For Univ 35(1):28–30

    Google Scholar 

Download references

Acknowledgments

This report was supported in part by funds provided by the Fulbright commission, funds from University of Illinois Agriculture Experiment Station project number 65-323, and funds from the Royal Thai government.

Author information

Authors and Affiliations

  1. Floriculture and Garden Design Department, Faculty of Agriculture, Alexandria University, Alexandria, Egypt

    A. M. Shehata

  2. Department of Natural Resources and Environmental Sciences, University of Illinois at Urbana-Champaign, Urbana, IL, 61801, USA

    W. Wannarat

  3. Department of Crop Sciences, University of Illinois at Urbana-Champaign, Urbana, IL, 61801, USA

    R. M. Skirvin & M. A. Norton

Authors
  1. A. M. Shehata
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. W. Wannarat
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. R. M. Skirvin
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. M. A. Norton
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to R. M. Skirvin.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Shehata, A.M., Wannarat, W., Skirvin, R.M. et al. The dual role of carbenicillin in shoot regeneration and somatic embryogenesis of horseradish (Armoracia rusticana) in vitro. Plant Cell Tiss Organ Cult 102, 397–402 (2010). https://doi.org/10.1007/s11240-010-9732-6

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s11240-010-9732-6

Keywords

Search

Navigation