Plant Cell, Tissue and Organ Culture (PCTOC)

, Volume 102, Issue 1, pp 129–134 | Cite as

Efficient regeneration and antioxidant potential in regenerated tissues of Piper nigrum L.

  • Nisar Ahmad
  • Hina Fazal
  • Bilal Haider AbbasiEmail author
  • Muhammad Rashid
  • Tariq Mahmood
  • Nighat Fatima
Research Note


The organogenic potential and antioxidant potential (1, 1-diphenyl-2-picrylhydrazyl-scavenging activity) of the medicinal plant Piper nigrum L. (black pepper) were investigated. Callus induction and shoot regeneration were induced from leaf explants of potted plants cultured on MS medium supplemented with different plant growth regulators. The best callogenic response was observed on explants cultured for 30 days on MS medium supplemented with either 0.5 or 1.5 mg l−1 6-benzyladenine (BA) + 1.0 mg l−1 α-naphthaleneacetic acid. Subsequent transfer of the callogenic explants onto MS medium supplemented with 1.5 mg l−1 BA + 1.0 mg l−1 gibberellic acid (GA3) achieved 85% shoot organogenesis after 30 days of culture. The maximum number (7.2) of shoots/explant was recorded for explants cultured in MS medium supplemented with 1.0 mg l−1 BA. Following the transfer of shoots to an elongation medium, the longest shoots (5.4 cm) were observed on MS medium supplemented with 1.0 mg l−1 BA + 1.0 mg l−1 GA3. The elongated shoots were rooted on MS medium supplemented with different concentrations of indole butyric acid. An assay of the antioxidant potential of the in vitro-grown tissues revealed that the antioxidant activity of the regenerated shoots was significantly higher than that of callus and the regenerated plantlets.


Antioxidant 6-Benzyladenine Gibberellic acid Organogenesis Piper nigrum 




2,4 D

2,4 Dichlorophenoxyacetic acid


1, 1-Diphenyl-2-picrylhydrazyl


Gibberellic acid


Indole butyric acid


MS medium without plant growth regulators


α-Naphthaleneacetic acid


Plant growth regulators



The authors acknowledge the support of Higher Education Commission (HEC) of Pakistan and University Research Fund (URF) of Quaid-i-Azam University (QAU), Islamabad, Pakistan.


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Copyright information

© Springer Science+Business Media B.V. 2010

Authors and Affiliations

  • Nisar Ahmad
    • 1
  • Hina Fazal
    • 2
    • 4
  • Bilal Haider Abbasi
    • 1
    Email author
  • Muhammad Rashid
    • 3
  • Tariq Mahmood
    • 2
  • Nighat Fatima
    • 1
  1. 1.Department of Biotechnology, Faculty of Biological SciencesQuaid-i-Azam UniversityIslamabadPakistan
  2. 2.Department of Plant SciencesQuaid-i-Azam UniversityIslamabadPakistan
  3. 3.Department of BotanyGomal UniversityDera Ismail KhanPakistan
  4. 4.Pakistan Council of Scientific and Industrial Research (PCSIR) Laboratories ComplexPeshawarPakistan

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