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Novel bioreactor technology for mass propagation of potato microtubers

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Abstract

Potato (Solanum tuberosum L. ssp. tuberosum) microtubers were produced in vitro with Liquid Lab™ Rocker system. A thin-layer liquid culture was applied together with a regular pitch in autoclavable simple plastic vessels. All cultures were carried out at room temperature without contamination problems. Each cultivar tested (Asterix, Timo, Van Gogh, Velox) formed microtubers in the Liquid Lab system. The mean number of microtubers per vessel (50 explants) varied between 30 (cv. Asterix in 8 weeks tuber induction) and 75 microtubers (cv. Velox in 11 weeks tuber induction). Majority (63%) of the microtubers was sufficient by size and weight (above 200 mg) for further storage at dormancy (4°C). The cv. Velox yielded the highest number of microtubers with cultivation capacity. As a result of prolonged microtuber induction of 2–3 weeks, more microtubers with competence for cultivation were obtained per cultivar, except for cv. Van Gogh. Still, the mean weight of Van Gogh microtubers was significantly higher after prolonged microtuber induction (0.67 g) than after short induction (0.51 g). In conclusion, Liquid Lab™ Rocker system is a novel, efficient and rapid system for mass propagation of potato.

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Fig. 1
Fig. 2

Abbreviations

L-L:

Liquid Lab™ Rocker

T.I.S.:

Temporary immersion system

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Acknowledgments

Northern Seed Potato Ltd. (Tyrnävä, Finland) is thanked for providing in vitro materials of potato cvs. Timo, Van Gogh, Asterix and Velox. The research was funded by the X-Branches Programme that belongs to the Innovative Action programmes supported by the ERDF, and implemented by MTT Agrifood Research Finland, Ruukki, and by Academy of Finland (113607).

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Authors and Affiliations

  1. Department of Biology, University of Oulu, P.O. Box 3000, 90014, Oulu, Finland

    T. Kämäräinen-Karppinen & A. M. Pirttilä

  2. MTT Agrifood Research Finland, Biotechnology and Food Research, Tutkimusasemantie 15, 92400, Ruukki, Finland

    E. Virtanen

  3. MTT Agrifood Research Finland, Biotechnology and Food Research, Myllytie 1, 31600, Jokioinen, Finland

    V.-M. Rokka

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  1. T. Kämäräinen-Karppinen
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  2. E. Virtanen
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  3. V.-M. Rokka
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  4. A. M. Pirttilä
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Correspondence to A. M. Pirttilä.

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Kämäräinen-Karppinen, T., Virtanen, E., Rokka, VM. et al. Novel bioreactor technology for mass propagation of potato microtubers. Plant Cell Tiss Organ Cult 101, 245–249 (2010). https://doi.org/10.1007/s11240-010-9679-7

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