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In vitro root culture of Ocimum sanctum L. and evaluation of its free radical scavenging activity

Abstract

Young leaf explants of Ocimum sanctum L. incubated on solidified Murashige and Skoog (MS) medium supplemented with 2 mg l−1 1-naphthaleneacetic acid (NAA) and 0.2 mg l−1 kinetin (Kn) developed rhizogenic callus. When these were subcultured onto MS medium supplemented with 1.5 mg l−1 2, 4-dichlorophenoxyacetic acid (2, 4-D) and 0.5 mg l−1 NAA, friable rhizogenic callus was observed. Upon transfer of this friable callus onto liquid MS medium containing 4 mg l−1 NAA and 1.3 mg l−1 6-benzyladnine (BA) under continuous agitation at 90 rpm and 16 h photoperiod, roots with an optimum dry weight of 1,460 mg l−1 were obtained. An ethyl acetate extract of these roots exhibited 1, 1–diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activity.

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Abbreviations

BA:

6-Benzyladnine.

2,4-D:

2,4-Dichlorophenoxyacetic acid.

DPPH:

1, 1–Diphenyl-2-picrylhydrazyl.

Kn:

Kinetin.

MS:

Murashige and Skoog.

NAA:

1-Naphthaleneacetic acid.

PGRs:

Plant growth regulators.

References

  • Begum F, Amin MN, Azad MAK (2002) In vitro rapid clonal propagation of Ocimum basilicum L. Plant Tissue Cult 12:27–35

    Google Scholar 

  • Blakesley D, Weston GD, Hall JF (1991) The role of endogenous auxin in root initiation. Part I. Evidence from studies on auxin application and analysis of endogenous levels. J Plant Growth Regul 10:341–353

    Article  CAS  Google Scholar 

  • Blosi MS (1958) Antioxidant determinations by the use of a stable free radical. Nature 181:1199–1200

    Article  Google Scholar 

  • Chatterjee A, Pakrashi SC (1997) The treatise of indian medicinal plants. 5th edn, pp 23–24, ISBN: 81-7236-179-3

  • Collin HA, Britton G (1993) Allium cepa L. (onion): In vitro culture and the production of flavor. In: Bajaj YPS (ed) Biotechnology in agriculture and forestry 24, Medicinal and aromatic plants V. Springer, Germany, pp 23–40

    Google Scholar 

  • Engelbrecht L, Mothes K (1961) The effect of kinetin on the development of roots. Plant Cell Physiol 2:271–276

    CAS  Google Scholar 

  • Farrukh A, Ahmad I, Mehmood Z (2006) Antioxidant and free radical scavenging properties of twelve traditionally used Indian medicinal plants. Turk J Biol 30:177–183

    Google Scholar 

  • Hybrid Brassica seed production. United States Patent 4499687

  • Mahajan UN, Wasule DD (2008) Sunscreen and anti-oxidant activities of herbal gel formulations. Phcog Mag suppl 4:99–101

    Google Scholar 

  • Maity TK, Mandal SC, Saha BP, Pal M (2000) Effect of Ocimum sanctum roots extract on swimming performance in mice. Phytother Res 14:120–121

    Article  CAS  PubMed  Google Scholar 

  • Mattsson J, Ckurshumova W, Berleth T (2003) Auxin Signaling in Arabidopsis Leaf Vascular Development. Plant Physiol 131:1327–1339

    Article  CAS  PubMed  Google Scholar 

  • Murashige T, Skoog F (1962) A revised medium for rapid growth and biossays with tobacco tissue cultures. Physiol Plant 15:473–497

    Article  CAS  Google Scholar 

  • Murata M, Orton TJ (1987) Callus initiation and regeneration capacities in Brassica species. Plant Cell Tissue Org Cult 11:111–123

    Article  Google Scholar 

  • Nabeta K (1993) Solanum aculeatissimum jacq: In vitro culture and the production of secondary metabolites. In: Bajaj YPS (ed) Biotechnology in agriculture and forestry 24, Medicinal and aromatic plants V. Springer, Germany, pp 329–341

    Google Scholar 

  • Nandagopal S, Kumari BDR (2007) Effectiveness of auxin induced in vitro root culture in chicory. J Cent Eur Agric 8:73–79

    Google Scholar 

  • Pattnaik S, Chand PK (1996) In vitro propagation of the medicinal herbs Ocimum americanum L. syn. O. canum Sims. (hoary basil) and Ocimum sanctum L. (holy basil). Plant Cell Rep 15(11):846–850

    Article  CAS  Google Scholar 

  • Samson J, Sheeladevi R, Ravindran R (2007) Oxidative stress in brain and antioxidant activity of Ocimum sanctum in noise exposure. NeuroToxicology 28:679–685

    Article  CAS  PubMed  Google Scholar 

  • Shetty K (2001) Biosynthesis and medical applications of rosmarinic acid. J Herbs Spices Med Plants 8:161–182

    Article  Google Scholar 

  • Singh B, Jaggi RK (2003) Antiinflammatory effect of Ocimum sanctum Linn and its cultures. Indian J Pharm Sci 65:425–428

    Google Scholar 

  • Singh NK, Sehgal CB (1999) Micropropagation of ‘Holy Basil’ (Ocimum sanctum Linn.) from young inflorescences of mature plants. J Plant Growth Regul 29:161–166

    Article  CAS  Google Scholar 

  • Sudha CG, Seeni S (2001) Establishment and analysis of fast-growing normal root culture of Decalepis arayalpathra, a rare endemic medicinal plant. Curr sci 81:371–374

    CAS  Google Scholar 

  • Tabata M, Fujitha Y (1985) Production of shikonin by plant cell cultures. In: Biotechnology in plant sciences. Academic press, New York, p 207

  • Washida D, Shimomura K, Takido M, Kitanaka S (2004) Auxins affected ginsenoside production and growth of hairy roots in Panax hybrid. Biol Pharm Bull 27:657–660

    Article  CAS  PubMed  Google Scholar 

  • Yeoman MM, Yeoman CL (1996) Tansley Review No 90, Manipulating secondary metabolism in cultured plant cells. New Phytol 134:553–569

    Article  CAS  Google Scholar 

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Correspondence to Baddireddi Subhadra Lakshmi.

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Shilpa, K., Selvakkumar, C., Senthil, A.K. et al. In vitro root culture of Ocimum sanctum L. and evaluation of its free radical scavenging activity. Plant Cell Tiss Organ Cult 101, 105–109 (2010). https://doi.org/10.1007/s11240-009-9661-4

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  • DOI: https://doi.org/10.1007/s11240-009-9661-4

Keywords

  • Ocimum sanctum
  • Normal root culture
  • 1-Naphthaleneacetic acid
  • 6-Benzyladnine
  • Free radical scavenging activity