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In vitro root culture of Ocimum sanctum L. and evaluation of its free radical scavenging activity


Young leaf explants of Ocimum sanctum L. incubated on solidified Murashige and Skoog (MS) medium supplemented with 2 mg l−1 1-naphthaleneacetic acid (NAA) and 0.2 mg l−1 kinetin (Kn) developed rhizogenic callus. When these were subcultured onto MS medium supplemented with 1.5 mg l−1 2, 4-dichlorophenoxyacetic acid (2, 4-D) and 0.5 mg l−1 NAA, friable rhizogenic callus was observed. Upon transfer of this friable callus onto liquid MS medium containing 4 mg l−1 NAA and 1.3 mg l−1 6-benzyladnine (BA) under continuous agitation at 90 rpm and 16 h photoperiod, roots with an optimum dry weight of 1,460 mg l−1 were obtained. An ethyl acetate extract of these roots exhibited 1, 1–diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activity.

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Fig. 1
Fig. 2





2,4-Dichlorophenoxyacetic acid.


1, 1–Diphenyl-2-picrylhydrazyl.




Murashige and Skoog.


1-Naphthaleneacetic acid.


Plant growth regulators.


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Correspondence to Baddireddi Subhadra Lakshmi.

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Shilpa, K., Selvakkumar, C., Senthil, A.K. et al. In vitro root culture of Ocimum sanctum L. and evaluation of its free radical scavenging activity. Plant Cell Tiss Organ Cult 101, 105–109 (2010).

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  • Ocimum sanctum
  • Normal root culture
  • 1-Naphthaleneacetic acid
  • 6-Benzyladnine
  • Free radical scavenging activity