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Estimation of the chlorophyll content of micropropagated potato plants using RGB based image analysis

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Abstract

A method has been developed for rapid and non invasive determination of chlorophyll content of leaves of micropropagated potato plants using RGB based image analysis. Among the trichomatic colors, R and G negatively correlated with the chlorophyll content, while a positive correlation was observed with B chromate. Compared to mean brightness value, the use of mean brightness ratio considerably improved the relationship of the tricolors with chlorophyll content. The brightness values and ratios of the primary colors are modeled as linear correlation functions for chlorophyll content. A significant correlation was observed between the model predicted chlorophyll content with the chlorophyll content measured by chlorophyll content meter. Spectral properties such as luminosity and saturation were also found to be negatively correlated with the chlorophyll content. The relationship was improved by combining the mean brightness ratio at B band region with luminosity. The potential of the imaging system in micropropagation has been discussed.

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Acknowledgments

The research was supported by grant (SP/INT/JAP/P-55/08) from Department of Science and Technology, New Delhi, India and Japan Society for Promotion of Science (JSPS) to Y.I. and S.D.G.

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Authors and Affiliations

  1. Agricultural and Food Engineering Department, Indian Institute of Technology Kharagpur, Kharagpur, 721302, India

    Satya Prakash Yadav & S. Dutta Gupta

  2. Department of Biological Science, Yamaguchi University, 1677-1 Yoshida, Yamaguchi, 753-8515, Japan

    Yasuomi Ibaraki

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  1. Satya Prakash Yadav
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  2. Yasuomi Ibaraki
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  3. S. Dutta Gupta
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Correspondence to S. Dutta Gupta.

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Yadav, S.P., Ibaraki, Y. & Dutta Gupta, S. Estimation of the chlorophyll content of micropropagated potato plants using RGB based image analysis. Plant Cell Tiss Organ Cult 100, 183–188 (2010). https://doi.org/10.1007/s11240-009-9635-6

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  • DOI: https://doi.org/10.1007/s11240-009-9635-6

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