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Intertribal somatic hybrids between Brassica napus and Camelina sativa with high linolenic acid content

Abstract

Intertribal somatic hybrids of Brassica napus and Camelina sativa were developed by protoplast electrofusion. Hybrid identity of the regenerants was determined using flow cytometric analysis of nuclear DNA content and simple sequence repeat (SSR) marker analysis. Three hybrids exhibited specific bands for B. napus and C. sativa. These hybrids showed intermediate leaf, flower and seed morphology compared with the two parental species. The seeds of these three hybrids had a modified fatty acid profile, indicating higher level of linolenic and eicosanoic acids than those of B. napus. Our results suggest that somatic hybridization offers opportunities for transferring entire genomes between B. napus and C. sativa in improving rapeseed breeding.

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Abbreviations

BA:

6-Benzyladenine

2,4-D:

2,4-Dichlorophenoxyacetic acid

FAME:

Fatty acid methyl ethers

MS:

Murashige and Skoog medium

NAA:

Naphthaleneacetic acid

PEG:

Polyethylene glycol

SSR:

Simple sequence repeats

TDZ:

Thidiazuron

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Acknowledgments

This work was funded by the National Key Project 973 (2006CB101603) and the Natural Science Foundation of Jiangsu Province (BK2007554, BK2008210).

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Correspondence to Y. P. Wang.

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J. J. Jiang and X. X. Zhao have equally contributed.

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Jiang, J.J., Zhao, X.X., Tian, W. et al. Intertribal somatic hybrids between Brassica napus and Camelina sativa with high linolenic acid content. Plant Cell Tiss Organ Cult 99, 91–95 (2009). https://doi.org/10.1007/s11240-009-9579-x

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  • DOI: https://doi.org/10.1007/s11240-009-9579-x

Keywords

  • Brassica napus
  • Camelina sativa
  • Protoplast fusion
  • Fatty acid alternation