Plant Cell, Tissue and Organ Culture (PCTOC)

, Volume 99, Issue 1, pp 27–34 | Cite as

Protoplast isolation and plant regeneration of different genotypes of Petunia and Calibrachoa

Original Paper


Protoplasts were isolated from leaves of in vitro-grown shoot cultures of different Petunia and Calibrachoa genotypes by enzyme digestion with 0.6% macerozyme R-10 and 2.0% cellulase R-10. Shoot regeneration was achieved in five out of nine Calibrachoa and three out of four Petunia genotypes. Protoplast yield and frequency of shoot regeneration varied among experiments and genotypes. Among all regenerants, few morphological changes, such as chlorophyll deficiency, variegated leaves, and polyploidization, were observed.


Protoplast yield Callus formation Plant regeneration and stability 





Fresh weight


Indole-3-acetic acid


Murashige and Skoog (1962)


1-Naphthaleneacetic acid




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Copyright information

© Springer Science+Business Media B.V. 2009

Authors and Affiliations

  1. 1.Institute of Ornamental and Woody Plant ScienceLeibniz University HannoverHannoverGermany

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