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Production of scopadulcic acid B from Scoparia dulcis Linn. using a Luffa sponge bioreactor

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Abstract

Production of Scopadulcic acid B (SDB), a diterpene having antiviral and anti-tumour activity, was accomplished by immobilizing suspension culture-derived cells of Scoparia dulcis on Luffa sponge matrix. The yield of SDB in shake flask cultures was 50.85 mg/g of cells after 30 days of incubation while, in Luffa sponge inoculated columns SDB production was scaled up to 350.57 mg/g of cells by the 19th day of the first batch operation. The trend was maintained up to the 22nd day and the half life period of the reactor was 10 days. The bioreactor with Luffa sponge is a novel system and can be exploited for the production of many pharmaceutically active natural compounds effectively.

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Abbreviations

NAA:

1 Naphthaleneacetic acid

BA:

N6-Benzylaminopurine

SDB:

Scopadulcic acid B

References

  • Akhtar N, Iqbal J, Iqbal M (2003) Microalgal-Luffa sponge immobilized disc: a new efficient biosorbant for removal of Ni (II) from aqueous solutions. Lett Appl Microbiol 37:49–153. doi:10.1046/j.1472-765X.2003.01366.x

    Article  Google Scholar 

  • Cheung IH, Gishen M, Ghosh P, Pamment NB (1986) Continuous ethanol production in an immobilized whole cell fermentor using untreated sugarcane baggase. Appl Microbiol Biotechnol 23:413–416. doi:10.1007/BF02346051

    Article  CAS  Google Scholar 

  • Gamble JS (1984) Flora of presidency of madras, vol 1. Allard & Sons, London, p 533

    Google Scholar 

  • Ganguly R, Dwivedi P, Singh RP (2007) Production of lactic acid with loofa sponge immobilized Rhizopus oryzae RBU2–10. Bioresour Technol 98:1246–1251. doi:10.1016/j.biortech.2006.05.004

    Article  PubMed  CAS  Google Scholar 

  • Georgiev V, Illieva M, Bley T, Pavolv A (2008) Betalain production in plant in vitro system. Acta Physiol Plant 30:581–593. doi:10.1007/s11738-008-0170-6

    Article  CAS  Google Scholar 

  • Hayashi T, Kishi M, Kawasaki M, Arisawa M, Shinizu M, Suzuki S, Yoshizaki M, Morita N, Tezuka Y, Kikuchi T, Bergena LH, Ferro E, Basualdo I (1987) Scopadulcic acid A and B, New diterpenoids with a novel skeleton, from a Paraguan crude drug Typachua Kuratu (Scoparia dulcis). Tetrahedron Lett 28:3693–3696. doi:10.1016/S0040-4039(00)96357-3

    Article  CAS  Google Scholar 

  • Hayashi T, Okamura K, Kakemi M, Asanos M, Mizutani M, Takeguchi N, Kawasaki M, Tezuka Y, Kikuchi T, Morita N (1990) Scopadulcic acid B, a new tetracyclic diterpenoid from S. dulcis L., its structures HT, K+ adenosine triphosphate inhibitory activity and pharmacokinetic behaviour in rats. Chem Pharm Bull (Tokyo) 38(274):2745

    Google Scholar 

  • Hayashi T, Okamura K, Kawasaki M, Morita N (1991) Two chemotypes of Scoparia dulcis in Paraguay. Phytochemistry 30:3617–3620. doi:10.1016/0031-9422(91)80078-F

    Article  CAS  Google Scholar 

  • Hayashi K, Niwayana S, Hayashi T, Nago R, Ochia H, Morita N (1998) In vitro and in vivo antiviral activity of scopadulcic acid B from S. dulcis, against herpes simplex virus type I. Antiviral Res 9:345–354. doi:10.1016/0166-3542(88)90036-8

    Article  Google Scholar 

  • Hoopen HJE, Gulik WM, Schaltman JE, Moreno PRH, Vinke JL, Heijen JJ, Verpoorte R (1994) Ajmaciline production by cell cultures of Catharanthus roseus from shake flask to bioreactors. Plant Cell Tissue Organ Cult 38:85–91. doi:10.1007/BF00033865

    Article  CAS  Google Scholar 

  • Iqbal M, Zafar SI (2008) Vegetable sponge as a matrix to immobilize microorganism a trial study for hyphal fungi, yeast and bacteria. Lett Appl Microbiol 18:214–217. doi:10.1111/j.1472-765X.1994.tb00850.x

    Article  Google Scholar 

  • Jayachandran K, Suresh PV, Chandrasekaran M (1994) A novel Acinetobacter sp for treating highly acidic rubber latex centrifugation effluent. Biotechnol Lett 16(6):649–654. doi:10.1007/BF00128616

    Article  CAS  Google Scholar 

  • Kirthikar KR, Basu BD (1933) Indian medicinal plants, vol 3. Allahabad Press, India, pp 1823–1824

    Google Scholar 

  • Kumar P, Satyanarayana T (2007) Production of thermostable and neutral glucoamylase using immobilized Thermomucor indicae seudaticae. World J Microbiol Biotechnol 23:509–517. doi:10.1007/s11274-006-9253-y

    Article  CAS  Google Scholar 

  • Liu YK, Seiki M, Tanaka H, Fursaki S (1998) Characteristics of Luffa sponge as a carrier for plant cell immobilization. J Ferment Bioeng 4:416–421. doi:10.1016/S0922-338X(98)80086-X

    Article  Google Scholar 

  • Min JY, Jung HY, Kang SM, Kim YD, Kang YM, Park DJ, Prasad DT (2007) Production of Tropane alkaloids by small scale bubble column bioreactor cultures of Scopalia parviflora adventitious roots. Bioresour Technol 98:1748–1753. doi:10.1016/j.biortech.2006.07.033

    Article  PubMed  CAS  Google Scholar 

  • Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with Tobacco tissue culture. Physiol Plant 15:473–497. doi:10.1111/j.1399-3054.1962.tb08052.x

    Article  CAS  Google Scholar 

  • Nishino H, Hayashi T, Arisawa M, Satomy Y, Iwashima A (1993) Antitumor promoting activity of Scopadulcic acid B, isolated from medicinal plant Scoparia dulcis L. Oncology 50:100–103

    Article  PubMed  CAS  Google Scholar 

  • Ogbonna JC, Chiu YC, Lui YK, Tanaka H (1994) Cell Immobilisation using Luffa sponge as a carrier. J Ferment Bioeng 78:437–442. doi:10.1016/0922-338X(94)90043-4

    Article  CAS  Google Scholar 

  • Ogbonna JC, Tomiyama S, Liu YC, Tanaka H (1997) Efficient production of ethanol by cells immobilized in loofa (Luffa cylindrica) sponge. J Ferment Bioeng 84:271–274. doi:10.1016/S0922-338X(97)82069-7

    Article  CAS  Google Scholar 

  • Roble ND, Ogbonna JC, Tanaka H (2004) I-Lactic acid production from raw Cassava starch in a circulating loop bioreactor in cells immobilized in Loofa (Luffa cylindrica). Biotechnol Lett 25:1093–1098. doi:10.1023/A:1024192131343

    Article  Google Scholar 

  • Santos DT, Sarrouh BF, Rivaldi JD, Converti A, Silvi SV (2007) Use of sugar cane baggasse as biomaterial for cell immobilization for xylitol production. J Food Eng 86:542–548. doi:10.1016/j.jfoodeng.2007.11.004

    Article  CAS  Google Scholar 

  • Zaho J, Verpoorte R (2007) Manipulating indole alkaloid production by Catharanthues roseus cell cultures in bioreactors: from biochemical processing to metabolic engineering. Phytochem Rev 6:435–457. doi:10.1007/s11101-006-9050-0

    Article  CAS  Google Scholar 

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Acknowledgments

The authors are grateful to the School of Biosciences, M. G. University, Kottayam, Kerala, India for providing the necessary facilities and for the financial support from the University Grants Commission, Bangalore (India). The first author acknowledges the institutional support of St. Thomas College, Kozhencherry, Kerala, India.

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Authors and Affiliations

  1. Department of Botany, St. Thomas College, Kozhencherry, Kerala, 689641, India

    Annie J. Mathew

  2. School of Biosciences, Mahatma Gandhi University, Kottayam, Kerala, 686560, India

    K. Jayachandran

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  1. Annie J. Mathew
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  2. K. Jayachandran
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Correspondence to K. Jayachandran.

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Mathew, A.J., Jayachandran, K. Production of scopadulcic acid B from Scoparia dulcis Linn. using a Luffa sponge bioreactor. Plant Cell Tiss Organ Cult 98, 197–203 (2009). https://doi.org/10.1007/s11240-009-9552-8

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  • DOI: https://doi.org/10.1007/s11240-009-9552-8

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