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Micropropagation of a Casuarina hybrid (Casuarina equisetifolia L. × Casuarina glauca Sieber ex Spreng) following facilitated seed germination

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Abstract

A suitable protocol for micropropagation of Casuarina hybrid, Casuarina equisetifolia L. × Casuarina glauca Sieber ex Spreng (C. e. × C. g.), was developed. When seeds without seed coats were cultured on 4 germination media, the optimal seed germination percentage (91%) was obtained on 0.8% agar solidified water medium. Shoot multiplication was achieved by culturing 2-cm long epicotyls, excised from germinated seedlings, on MS (Murashige and Skoog 1962) basal medium supplemented with BA (6-benzylaminopurine) at 4.4, 8.8, 17.8 and 35.6 μM. The greatest percentage of axillary bud sproutings (87.5%), mean number of sprouts per explant (3.8), and shoot length (3.2 cm) were achieved on MS medium supplemented with 17.8 μM BA. MS medium supplemented with 4 different concentrations of IBA (indole-3-butyric acid) (4.3, 8.7, 13.0 and 17.4 μM) were used for rooting of in vitro grown shoots. The highest rooting percentage (65.6%), mean number of roots per explant (2.5) and mean length of roots per explant (1.6 cm) was achieved at 13.0 μM IBA. Rooted shoots grew well after transfer to a substrate of peat and pinebark (7:3) in the greenhouse.

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Abbreviations

BA:

6-Benzylaminopurine

C. e. × C. g. :

Casuarina equisetifolia L. × Casuarina glauca Sieber ex Spreng

IBA:

Indole-3-butyric acid

MS:

Murashige and Skoog (1962)

PGRs:

Plant growth regulators

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Authors and Affiliations

  1. Citrus Research and Education Center (CREC), Institute of Food and Agricultural Sciences (IFAS), University of Florida (UF), 700 Experiment Station Road, Lake Alfred, FL, 33850-2299, USA

    Xiuli Shen, William S. Castle & Frederick G. Gmitter Jr

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  1. Xiuli Shen
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  2. William S. Castle
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  3. Frederick G. Gmitter Jr
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Correspondence to William S. Castle.

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Shen, X., Castle, W.S. & Gmitter, F.G. Micropropagation of a Casuarina hybrid (Casuarina equisetifolia L. × Casuarina glauca Sieber ex Spreng) following facilitated seed germination. Plant Cell Tiss Organ Cult 97, 103–108 (2009). https://doi.org/10.1007/s11240-009-9502-5

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