Abstract
Plant tissue cultures represent a potential source for producing secondary metabolites. In this work, Buddleja cordata tissue cultures were established in order to produce phenylpropanoids (verbascoside, linarin and hydroxycinnamic acids), as these metabolites are credited with therapeutic properties. Highest callus induction (76.4–84.3%) was obtained in five treatments containing 2,4-Dichlorophenoxyacetic acid (2,4-d: 0.45–9.05 μM) with Kinetin (KIN: 2.32, 4.65 μM), whereas highest root induction (79.6%) corresponded to the α-Naphthaleneacetic acid (9.05 μM) with KIN (2.32 μM) treatment. Verbascoside was the major phenylpropanoid produced in in vitro cultures (root, white and green callus) [66.24–86.26 mg g−1 dry weight (DW)], while linarin and hydroxycinnamic acid production was low (0.95–3.01 mg g−1 DW). Verbascoside and linarin production were improved in cell suspension culture (116 mg g−1 DW and 8.12 mg g−1 DW, respectively).
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Abbreviations
- ANOVA:
-
Analysis of variance
- DAD:
-
Photodiode array detection
- DW:
-
Dry weight
- GI:
-
Growth index
- HPLC:
-
High performance liquid chromatography
- IAA:
-
Indole-3-acetic acid
- KIN:
-
Kinetin
- μ:
-
Specific growth rate
- MB:
-
Maximum biomass
- NAA:
-
α-Naphthaleneacetic acid
- PGR(s):
-
Plant growth regulator(s)
- qp:
-
Specific production rate
- td:
-
Doubling time
- Y X/S :
-
Yield of biomass from substrate
- 2,4-d :
-
2,4-Dichlorophenoxyacetic acid
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Acknowledgements
The authors thank the Programa de Apoyo de la Investigación Multidisciplinario (Desarrollo de fitomedicamentos a partir de plantas medicinales y sus extractos para el control de la diabetes mellitus) for the partial financing of this project, and the Consejo Nacional de Ciencia y Tecnología (CONACyT) through grant 81157. We also thank Biologist Jorge Santana Carrillo at UAM-I herbarium for taxonomic identification of the collected plant as Buddleja cordata.
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Estrada-Zúñiga, M.E., Cruz-Sosa, F., Rodríguez-Monroy, M. et al. Phenylpropanoid production in callus and cell suspension cultures of Buddleja cordata Kunth. Plant Cell Tiss Organ Cult 97, 39–47 (2009). https://doi.org/10.1007/s11240-009-9496-z
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DOI: https://doi.org/10.1007/s11240-009-9496-z