Genetic transformation of golden pothos (Epipremnum aureum) mediated by Agrobacterium tumefaciens
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To establish a procedure for Agrobacterium tumefaciens-mediated transformation of golden pothos (Epipremnum aureum) plants, the effects of selection antibiotics and the preculture period of stem explants before A. tumefaciens infection were examined. Explants were co-cultivated with A. tumefaciens EHA105, harboring the plasmid pGWB2/cGUS, on a somatic embryo-inducing medium supplemented with acetosyringone. Resulting transgenic somatic embryos were screened on an antibiotic selection medium, and the transgenic pothos plants were regenerated on a germination medium. Hygromycin was the optimum selection antibiotic tested. The preculture period significantly affected the transformation efficiency, with explants precultured for one-day showing the best efficiency (5–30%). Both transformed hygromycin-resistant embryos and regenerated plants showed β-glucuronidase activity. Southern blot analysis confirmed transgene integration into the pothos genome. This reproducible transformation system for golden pothos may enable the molecular breeding of this very common indoor plant.
KeywordsGolden pothos Transgenic plants Somatic embryo Agrobacterium tumefaciens
Cauliflower mosaic virus 35S promoter
Murashige and Skoog
α-Naphthalene acetic acid
Polymerase chain reaction
We thank Dr. Shibata for providing the plasmid pGWB2/cGUS.
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