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Plant regeneration from in vitro leaves of mature black cherry (Prunus serotina)

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Abstract

A regeneration system was developed for Prunus serotina from a juvenile (F) and two mature genotypes (#3 and #4). Adventitious shoots regenerated from leaves of in vitro cultures on woody plant medium with thidiazuron (TDZ) and naphthaleneacetic acid (NAA). The best regeneration for genotype F (91.4%) was observed on medium with 9.08 μM TDZ and 1.07 μM NAA. The highest mean number of shoots (8.2) was obtained on medium containing 9.08 μM TDZ and 0.54 μM NAA. Genotype #3 had the highest regeneration (41.7%) with a mean number of shoots (4.8) on 9.08 μM TDZ and 1.07 μM NAA, whereas genotype #4 had a 38.8% regeneration with a mean of 3.3 shoots. Genotype #4 had the highest mean number of shoots (4.8) on 4.54 μM TDZ and 1.07 μM NAA. Silver thiosulphate at 60 or 80 μM increased the percent regeneration of the mature genotypes #3 (75%) and #4 (58%). Adventious shoots were rooted (70–76%) and rooted plantlets survived after acclimatization to the greenhouse. The effect of kanamycin concentration on adventitious shoot regeneration was also evaluated.

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Abbreviations

BA:

6-benzylaminopurine

GA3 :

Gibberellic acid

IBA:

Indole-3-butyric acid

MS:

Murashige and Skoog medium

NAA:

Napthaleneacetic acid

STS:

Silver thiosulphate

TDZ:

Thidiazuron

WPM:

Woody plant medium

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Acknowledgements

The authors wish to thank Dr. Stanton Gelvin (Purdue University) for providing the avirulent strain of A. tumefaciens. The authors gratefully acknowledge Drs. Valerie Pence and Barbara Reed for their constructive review and suggestions for improvement of this manuscript. Mention of a trademark, proprietary product, or vendor does not constitute a guarantee or warranty of the product by the US Department of Agriculture and does not imply its approval to the exclusion of other products or vendors that also may be suitable.

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Authors and Affiliations

  1. Department of Forestry and Natural Resources, Hardwood Tree Improvement and Regeneration Center (HTIRC), Purdue University, 715 West State Street, West Lafayette, IN, 47907, USA

    Xiaomei Liu

  2. USDA Forest Service, Northern Research Station, HTIRC, 715 West State Street, West Lafayette, IN, 47907, USA

    Paula M. Pijut

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  1. Xiaomei Liu
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  2. Paula M. Pijut
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Correspondence to Paula M. Pijut.

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Liu, X., Pijut, P.M. Plant regeneration from in vitro leaves of mature black cherry (Prunus serotina). Plant Cell Tiss Organ Cult 94, 113–123 (2008). https://doi.org/10.1007/s11240-008-9393-x

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  • DOI: https://doi.org/10.1007/s11240-008-9393-x

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