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Stress-induced changes important for effective androgenic induction in isolated microspore culture of triticale (×Triticosecale Wittm.)

Plant Cell, Tissue and Organ Culture volume 94, pages 319–328 (2008)Cite this article

Abstract

The accumulation of abscisic acid (ABA) and the activities of antioxidative enzymes along with cell metabolic activity were monitored during androgenesis induction in triticale (×Triticosecale Wittm.). Tested cultivars ‘Mieszko’ and ‘Wanad’ were selected due to their significantly different responses to androgenic induction. Significant variation was observed in respect of superoxide dismutase activity and endogenous ABA content in anthers isolated from freshly cut tillers. For both cultivars, tillers pretreatment with low temperature decreased peroxidase activity by 36%, highly accelerated respiration rate and reduced heat production. At the same time, the level of ABA in ‘Mieszko’ was increased to the level measured in ‘Wanad’. This effect was associated with higher microspore culture viability and increased stress tolerance in ‘Mieszko’. Low temperature and metabolic starvation during 4-day anther preculture did not influence activities of antioxidative enzymes, while it resulted in slight decrease in respiration rate and heat emission. The importance of these changes for effective androgenesis induction is discussed.

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Abbreviations

ABA:

Abscisic acid

CAT:

Catalase

CPT:

Anthers isolated from cold pretreated tillers

DH:

Doubled haploids

DW:

Dry weight

ELS:

Embryo-like structures

FCT:

Anthers isolated from freshly cut tillers

FDA:

Fluorescein diacetate

NAA:

α-Naphthaleneacetic acid

PC:

Precultured anthers

PEX:

Peroxidase

ROS:

Reactive oxygen species

SOD:

Superoxide dismutase

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Acknowledgement

The research was supported by the project KBN23/E189/SPB/COST/P06/Dz585/2002-2005.

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Authors and Affiliations

  1. Institute of Plant Physiology Polish Academy of Sciences, Niezapominajek 21, Krakow, 30-239, Poland

    Iwona Żur, Ewa Dubas, Elżbieta Golemiec, Magdalena Szechyńska-Hebda, Franciszek Janowiak & Maria Wędzony

  2. Pedagogical University of Kraków, Podchorążych 2, Krakow, 30-084, Poland

    Maria Wędzony

Authors
  1. Iwona Żur
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  2. Ewa Dubas
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Correspondence to Iwona Żur.

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Żur, I., Dubas, E., Golemiec, E. et al. Stress-induced changes important for effective androgenic induction in isolated microspore culture of triticale (×Triticosecale Wittm.). Plant Cell Tiss Organ Cult 94, 319–328 (2008). https://doi.org/10.1007/s11240-008-9360-6

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Keywords

  • Abscisic acid
  • Antioxidative enzymes
  • Metabolic activity
  • Oxidative stress
  • Androgenesis