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Plant Cell, Tissue and Organ Culture

, Volume 94, Issue 3, pp 319–328 | Cite as

Stress-induced changes important for effective androgenic induction in isolated microspore culture of triticale (×Triticosecale Wittm.)

  • Iwona ŻurEmail author
  • Ewa Dubas
  • Elżbieta Golemiec
  • Magdalena Szechyńska-Hebda
  • Franciszek Janowiak
  • Maria Wędzony
Original Paper

Abstract

The accumulation of abscisic acid (ABA) and the activities of antioxidative enzymes along with cell metabolic activity were monitored during androgenesis induction in triticale (×Triticosecale Wittm.). Tested cultivars ‘Mieszko’ and ‘Wanad’ were selected due to their significantly different responses to androgenic induction. Significant variation was observed in respect of superoxide dismutase activity and endogenous ABA content in anthers isolated from freshly cut tillers. For both cultivars, tillers pretreatment with low temperature decreased peroxidase activity by 36%, highly accelerated respiration rate and reduced heat production. At the same time, the level of ABA in ‘Mieszko’ was increased to the level measured in ‘Wanad’. This effect was associated with higher microspore culture viability and increased stress tolerance in ‘Mieszko’. Low temperature and metabolic starvation during 4-day anther preculture did not influence activities of antioxidative enzymes, while it resulted in slight decrease in respiration rate and heat emission. The importance of these changes for effective androgenesis induction is discussed.

Keywords

Abscisic acid Antioxidative enzymes Metabolic activity Oxidative stress Androgenesis 

Abbreviations

ABA

Abscisic acid

CAT

Catalase

CPT

Anthers isolated from cold pretreated tillers

DH

Doubled haploids

DW

Dry weight

ELS

Embryo-like structures

FCT

Anthers isolated from freshly cut tillers

FDA

Fluorescein diacetate

NAA

α-Naphthaleneacetic acid

PC

Precultured anthers

PEX

Peroxidase

ROS

Reactive oxygen species

SOD

Superoxide dismutase

Notes

Acknowledgement

The research was supported by the project KBN23/E189/SPB/COST/P06/Dz585/2002-2005.

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Copyright information

© Springer Science+Business Media B.V. 2008

Authors and Affiliations

  • Iwona Żur
    • 1
    Email author
  • Ewa Dubas
    • 1
  • Elżbieta Golemiec
    • 1
  • Magdalena Szechyńska-Hebda
    • 1
  • Franciszek Janowiak
    • 1
  • Maria Wędzony
    • 1
    • 2
  1. 1.Institute of Plant Physiology Polish Academy of SciencesKrakowPoland
  2. 2.Pedagogical University of KrakówKrakowPoland

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