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Callus induction and shoot regeneration of Phaseolus lunatus L. cv. Wonder Bush and cv. Pole Sieva

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Abstract

We report the first protocol for callus induction and shoot regeneration for Phaseolus lunatus L. cv. Wonder Bush and cv. Pole Sieva. We used different explants viz., epicotyls, cotyledons and hypocotyls. The medium used was MS basal medium with thidiazuron (0.5 mg l−1) and IAA (0.05 mg l−1) for the induction of callus followed by BAP (1.0 mg l−1) for the induction of shoots. Epicotyl explants showed the fastest response and the highest percentage of shoot regeneration. This protocol opens new biotechnological strategies to transfer economically important genes to this important crop species.

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Abbreviations

TDZ:

Thidiazuron (1-phenyl-3-(1,2,3-thiadiazol-5-yl) urea)

BAP:

6-Benzylaminopurine

IAA:

Indole-3-acetic acid

CIM:

Callus induction medium

SIM:

Shoot induction medium

SDM:

Shoot development medium

MS:

Murashige and Skoog

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Authors and Affiliations

  1. Department of Plant Biology and Centre of Excellence CEBIOVEM, University of Turin, Viale P.A. Mattioli 25, 10125, Turin, Italy

    Chidananda Nagamangala Kanchiswamy & Massimo Maffei

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  1. Chidananda Nagamangala Kanchiswamy
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  2. Massimo Maffei
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Correspondence to Massimo Maffei.

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Kanchiswamy, C.N., Maffei, M. Callus induction and shoot regeneration of Phaseolus lunatus L. cv. Wonder Bush and cv. Pole Sieva. Plant Cell Tiss Organ Cult 92, 239–242 (2008). https://doi.org/10.1007/s11240-007-9322-4

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  • DOI: https://doi.org/10.1007/s11240-007-9322-4

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