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Effect of red- and blue-light-emitting diodes on growth and morphogenesis of grapes

Abstract

The effect of red- and blue-light-emitting diodes on shoot and root growth of Hybrid Franc grape, a rootstock cultivar, along with two other grape genotypes, Kadainou R-1 and Vitis ficifolia var. ganebu, were investigated in vitro. Plants cultured under red-light-emitting diodes produced the longest shoots with longer internodes for all genotypes. The chlorophyll content measured as SPAD value and leaf number per explant were highest on plants cultured under blue-light-emitting diodes in all the genotypes. Blue light was also responsible for a higher number of stomata in all the genotypes; however, there was no significant difference in size of stomata in all genotypes under the different light conditions tested. Different light-emitting diodes did not affect the rooting percentage of Hybrid Franc but red-light-emitting diodes gave a higher rooting percentage along with higher root numbers for the two other grape genotypes.

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Abbreviations

B-LEDs:

Blue-light-emitting diodes

IAAO:

IAA oxidase

LEDs:

Light-emitting diodes

R-LEDs:

Red-light-emitting diodes

PGF:

Plant growth fluorescent

PGR:

Plant growth regulator

NAA:

α-Naphthalene acetic acid

References

  • Akoyunoglou G, Anni H (1984) Blue light effect on chloroplast development in higher plants. In: Senger H (ed) Blue light effects in biological systems, Springer Verlag, Berlin, pp 397–406

    Google Scholar 

  • Aksenova NP, Konstantinova TN, Sergeeva LI, Macháčková I, Golyanovskaya SA (1994) Morphogenesis of potato plants in vitro. I. Effect of light quality and hormones. J Plant Growth Reg 13:143–146

    Article  CAS  Google Scholar 

  • Barlass M, Skene KGM (1978) In vitro propagation of grapevine (Vitis vinifera L.) from fragmented shoot apices. Vitis 17:335–340

    Google Scholar 

  • Bula RJ, Morrow RC, Tibbitts TW, Barta DJ, Ignatius RW, Martin TS (1991) Light-emitting diodes as a radiation source for plants. HortScience 26(2):203–205

    PubMed  CAS  Google Scholar 

  • Chée R (1986) In vitro culture of Vitis: the effects of light spectrum, manganese sulfate and potassium iodide on morphogenesis. Plant Cell Tiss Org Cult 1:121–134

    Article  Google Scholar 

  • Chée R, Pool RM (1982) The effect of growth substances and photoperiod on the development of shoot apices of Vitis cultured in vitro. Sci Hort 16:17–27

    Article  Google Scholar 

  • Cheng ZM, Reisch BI (1989) Shoot regeneration from petiole and leaves of Vitis ×  labruscana ‘Catawba’. Plant Cell Rep 8:403–406

    Article  CAS  Google Scholar 

  • Goins GD, Yorio NC, Sanwo MM, Brown CS (1997) Photomorphogenesis, photosynthesis, and seed yield of wheat plants grown under red light-emitting diodes (LEDs) with and without supplemental blue lighting. J Exp Bot 48(312):1407–1413

    PubMed  Article  CAS  Google Scholar 

  • Goldy S, Goldy RG (1991) Growth regulator and axillary bud position effects on in vitro establishment of Vitis rotundifolia. HortScience 26(3):304–307

    Google Scholar 

  • Gray DJ, Benton CM (1991) In vitro micropropagation and plant establishment of muscadine grape cultivars (Vitis rotundifolia). Plant Cell Tiss Org Cult 21:7–14

    Article  Google Scholar 

  • Gribaudo I, Fronda A (1991) Effects of thidiazuron on grapevine axillary buds cultivated in vitro. HortScience 26(8):1083

    Google Scholar 

  • Heloir M, Fournioux JC, Oziol L, Bessis R (1997) An improved procedure for the propagation in vitro of grapevine (Vitis vinifera cv. Pinot noir) using axillary-bud microcuttings. Plant Cell Tiss Org Cult 49:223–225

    Article  CAS  Google Scholar 

  • Heo J, Lee C, Chakrabarty D, Paek K (2002) Growth responses of marigold and salvia bedding plants as affected by monochromic or mixture radiation provided by a light-emitting diode (LED). Plant Growth Regul 38:225–230

    Article  CAS  Google Scholar 

  • Hughes KW (1981) In vitro ecology: exogenous factors affecting growth and morphogenesis in plant culture systems. Environ Expt Bot 21:281–288

    Article  CAS  Google Scholar 

  • Ibáñez A, Valero M, Morte A (2003) Influence of cytokinins and subculturing on proliferation capacity of single-axillary-bud microcuttings of Vitis vinifera L. cv. Napoleon. Anales de Biologia 25:81–90

    Google Scholar 

  • Jao RC, Lai CC, Fang W, Chang SF (2005) Effects of red light on the growth of Zantedeschia plantlets in vitro and tuber formation using light-emitting diodes. HortScience 40(2):436–438

    Google Scholar 

  • Kim S, Hahn EJ, Heo JW, Paek KY (2004) Effect of LEDs on net photosynthetic rate, growth and leaf stomata of chrysanthemum plantlets in vitro. Sci Hort 101:143–151

    Article  Google Scholar 

  • Lian ML, Murthy HN, Paek KY (2002) Effect of light emitting diodes (LEDs) on the in vitro induction and growth of bulblets of Lilium oriental hybrid ‘Pesaro’. Sci Hort 94:365–370

    Google Scholar 

  • Moreira da Silva MH, Debergh PC (1997) The effect of light quality on the morphogenesis of in vitro cultures of Azorina vidalii (Wats.) Feer. Plant Cell Tiss Org Cult 51:187–193

    Article  Google Scholar 

  • Mumford FE, Smith DH, Castle JE (1961) An inhibitor of indoleacetic acid oxidase from pea tips. Plant Physiol 36:752–756

    PubMed  CAS  Article  Google Scholar 

  • Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue culture. Physiol Plant 15:473–497

    Article  CAS  Google Scholar 

  • Nhut DT, Takamura T, Watanabe H, Murakami A, Murakami K, Tanaka M (2002) Sugar-free micropropagation of Eucalyptus citriodora using light-emitting diode (LEDs) and film-rockwool culture system. Environ Control Biol 40(2):147–155

    Google Scholar 

  • Novák FJ, Jůvová Z (1982/83) Clonal propagation of grapevine through in vitro axillary bud culture. Sci Hort 18:231–240

    Google Scholar 

  • Okamoto K, Yanagi T, Kondo S (1997) Growth and morphogenesis of lettuce seedlings raised under different combinations of red and blue light. Acta Hort 445:149–157

    Google Scholar 

  • Pool RM, Powell LE (1975) The influence of cytokinins on in vitro shoot development of ‘Concord Grape’. J Amer Soc Hort Sci 100(2):200–202

    CAS  Google Scholar 

  • Poudel PR, Kataoka I, Mochioka R (2005) Effect of plant growth regulators on in vitro propagation of Vitis ficifolia var. ganebu and its interspecific hybrid grape. Asian J Plant Sci 4(5):466–471

    CAS  Google Scholar 

  • Richter G, Wessel K (1985) Red light inhibits blue-induced chloroplast development in cultured plant cells at the mRNA level. Plant Mol Biol 5:175–182

    Article  CAS  Google Scholar 

  • Schuerger AC, Brown CS, Stryjewski EC (1997) Anatomical features of Pepper plants (Capsicum annum L.) grown under red light-emitting diodes supplemented with blue or far-red light. Ann Bot 79:273–282

    PubMed  Article  CAS  Google Scholar 

  • Singh SK, Khawale RN, Singh SP (2004) Techniques for rapid in vitro propagation of Vitis vinifera L. cultivars. J Hort Sci Biotech 79(2):267–272

    Google Scholar 

  • Tanaka M, Takamura T, Watanabe H, Endo M, Yanagi T, Okamoto K (1998) In vitro growth of cymbidium plantlets cultured under super bright and blue light-emitting diodes (LEDs). J Hort Sci Biotech 73(1):39–44

    Google Scholar 

  • Tennessen DJ, Singsaas EL, Sharkey TD (1994) Light-emitting diodes as a source for photosynthesis research. Photosynth Res 39:85–92

    Article  CAS  Google Scholar 

  • Topchiy NM, Sytnik SK, Syvash OO, Zolotareva OK (2005) The effect of additional red irradiation on the photosynthetic apparatus of Pisum sativum. Photosynthetica 43(3):451–456

    Article  CAS  Google Scholar 

  • Vinterhalter D, Grubišić D, Vinterhalter B, Konjević R (1990) Light controlled root elongation in in vitro cultures of Dracaena fragrans Ker-Gawl. Plant Cell Tiss Org Cult 22:1–6

    Google Scholar 

  • Yanagi T, Okamoto K, Takita S (1996) Effect of blue, red and blue/red lights on two different PPF levels on growth and morphogenesis of lettuce plants. Acta Hort 440:117–122

    CAS  Google Scholar 

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Correspondence to Puspa Raj Poudel.

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Poudel, P.R., Kataoka, I. & Mochioka, R. Effect of red- and blue-light-emitting diodes on growth and morphogenesis of grapes. Plant Cell Tiss Organ Cult 92, 147–153 (2008). https://doi.org/10.1007/s11240-007-9317-1

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  • DOI: https://doi.org/10.1007/s11240-007-9317-1

Keywords

  • Hybrid Franc
  • In vitro
  • Light quality
  • Shoot growth
  • Propagation