Abstract
Light irradiation had remarkable effects on callus growth of Oldenlandia affinis with an optimum intensity of 35 μmol m−2 s−1. Biosynthesis of kalata B1, the main cyclic peptide in O. affinis, was induced and triggered with rising irradiation intensities. The highest concentration of kalata B1, 0.49 mg g−1 DW characterised by the maximum productivity of 3.88 μg per litre and day was analysed at 120 μmol m−2 s−1, although callus growth was repressed. The light saturation point was established to be 35 μmol m−2 s−1, where kalata B1 productivity was in a similar order (3.41 μg per day) due to the higher growth index. O. affinis suspension cultures were shown to accumulate comparable specific kalata B1 concentrations in a delayed growth associated production pattern. These were dependent on irradiation intensity (0.16 mg g−1 at 2 μmol m−2 s−1; 0.28 mg g−1 at 35 μmol m−2 s−1). The batch cultivation process resulted in a maximum productivity of 27.30 μg per litre and day with culture doubling times of 1.16 d−1. Submers operation represented a 8-fold product enhancement compared to callus cultivation.
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This study was supported by a Research Grant of the Deutsche Forschungsgemeinschaft DFG (DO 550/5-1).
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Dörnenburg, H., Seydel, P. Effect of irradiation intensity on cell growth and kalata B1 accumulation in Oldenlandia affinis cultures. Plant Cell Tiss Organ Cult 92, 93–99 (2008). https://doi.org/10.1007/s11240-007-9309-1
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DOI: https://doi.org/10.1007/s11240-007-9309-1