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The use of excised roots from in vitro culture for the determination of superoxide production in plants

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Abstract

The production of reactive oxygen species (ROS) in plants is a common event in metabolic and physiological processes as well as in the response to biotic and abiotic stress. In this paper we will report that root tissue from axenically grown tomato cultivars and Lycopersicon wild species can be used for the determination of superoxide production. Superoxide generation was evaluated following the treatment of root tissues with two general elicitors of the defence response: laminarin and calcium ionophore A23187. Results demonstrated that elicitor reactivity in terms of superoxide generation of the tomato cultivars and the wild species used was different. This suggested varying levels of competence for non-specific active defence. The proposed technique merges the advantages of in vitro cultures and of whole tissues and also demonstrates that root tissue is a suitable material for evaluating free radical release.

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Abbreviations

ANOVA:

Analysis of variance

CAT:

Catalase

Cyt c :

Cytochrome c

HPRGs:

Hydroxyproline-rich glycoproteins

HR:

Hypersensitive response

LS:

Linsmajer and Skoog medium

NADPH:

Nicotinamide adenine dinucleoside phosphate

ROI:

Reactive oxygen intermediates

ROS:

Reactive oxygen species

SOD:

Superoxide dismutase

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Acknowledgements

This Research was funded by the Italian Ministry of Agricultural and Forest Policies, project ‘Plant protection using molecular markers (PROMAR). Authors are grateful to Prof. A Scala (Department of Agricultural Biotechnology, University of Florence, Italy) for helpful discussion and critical reading of the manuscript, to Prof. FM Stefanini (Department of Statistics ‘Giuseppe Parenti’, University of Florence, Italy) for critical discussion on statistical analysis and to Dr. M Halperin for revising the English form of the manuscript.

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Authors and Affiliations

  1. Dipartimento di Biologia animale e Genetica ‘Leo Pardi’, Università degli Studi di Firenze, Via Romana 17-19, 50125, Florence, Italy

    E. Cosi, P. Bettini, D. Bindi & M. Buiatti

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  1. E. Cosi
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  2. P. Bettini
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  3. D. Bindi
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  4. M. Buiatti
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Correspondence to M. Buiatti.

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Cosi, E., Bettini, P., Bindi, D. et al. The use of excised roots from in vitro culture for the determination of superoxide production in plants. Plant Cell Tiss Organ Cult 89, 203–209 (2007). https://doi.org/10.1007/s11240-007-9239-y

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  • DOI: https://doi.org/10.1007/s11240-007-9239-y

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