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Interspecific hybridization of Prunus persica with P. armeniaca and P. salicina using embryo rescue

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Abstract

Embryo rescue technique was used successfully to produce interspecific hybrids by crossing peach (P. persica) as a female parent with apricot (P. armeniaca) and plum (P. salicica). In those crosses that had ‘Yuhualu’ or ‘Zhonghuashoutao’ as female parents, hybrid embryos aborted from the 7th or 8th week after pollination mainly due to post-pollination incompatibility. An embryo rescue protocol was established to rescue such embryos and recover hybrid plants. Modified half-strength MS medium containing 4 mg l−1 6-BA and 0.5 mg l−1 IBA produced up to 90% germination in the embryos. Modified MS medium with 1.0 mg l−1 6-BA and 1.0 mg l−1 IBA gave the highest bud induction and multiplication whereas modified MS medium containing 0.5 mg l−1 IAA and 0.2 mg l−1 NAA gave the best rooting percentage. All the hybrids obtained using this embryo rescue technique were verified using simple sequence repeat (SSR) markers. A series of pollen treatments were carried out to partially overcome pre-pollination incompatibility, and it was found accidentally that pollen treatment with electrostatic field not only improved pollen germination but also increased the multiplication coefficient of embryo-induced shoots.

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Abbreviations

IAA:

indole-3-acetic acid

IBA:

indole-3-butyric acid

NAA:

α-naphthaleneacetic acid

6-BA:

6-benzyladenine

MS:

Murashige and Skoog (1962) medium

PF:

embryo length/seed length

SSR:

simple sequence repeat

References

  • Adu-Amphomah Y, Novak FJ, Klu GYP, Lamptey TVO (1991) Use of irradiated pollen as mentor pollen to induce self-fertilization of two self-incompatible Upper Amazon Cacao clones. Euphytica 51:219–225

    Article  Google Scholar 

  • Alberto CQP, Pinto Q, Suzanne M, David H (1994) Growth of immature peach embryos in response to media, ovule support method, and ovule perforation. Hortic Sci 29:1081–1083

    Google Scholar 

  • Boavida LC, Silva JP, Feījó JA (2001) Sexual reproduction in the cork oak (Quercus suber L). II. Crossing intra- and interspecific barriers. Sex Plant Reprod 14:143–152

    Article  Google Scholar 

  • Bouvier L, Zhang YX, Lespinasse Y (1993) Two methods of haploidization in pear, Pyrus communis L.: greenhouse seedling selection and in situ parthenogenesis induced by irradiated pollen. Theor Appl Genet 87:229–232

    Article  Google Scholar 

  • Chen J, Staub J, Qian Ch, Jiang J, Luo X, Zhuang F (2003) Reproduction and cytogenetic characterization of interspecific hybrids derived from Cucumis hystrix Chakr. × Cucumis sativus L. Theor Appl Genet 106:688–695

    PubMed  CAS  Google Scholar 

  • Chopra HR, Kanwar JS, Gosal SS (1994) Embryo culture as an effective aid for rescuing hybrid seedlings of early ripening peaches (Prunus persica Batsch). J Res Punjab Agric Univ 31:127–130

    Google Scholar 

  • Crance JC (1969) The role of hormones in fruit set and development. Hortic Sci 4:108–111

    Google Scholar 

  • Daskalov H, Konsztantinova M, Daskalov KH (1979) The effect of ultraviolet and gamma irradiation on anthers, on heterosis in the F1 and on the possibility of distant hybridization. Kerteszeti-Egyetem-Kozlemenyei 42:23–34

    Google Scholar 

  • Dirlewanger E, Cosson P, Tavaud M, Aranzana MJ, Poizat C, Zanetto A, Arús P, Laigret F (2002) Development of microsatellite markers in peach [Prunus persica (L.) Batsch] and their use in genetic diversity analysis in peach and sweet cherry (Prunus avium L.). Theor Appl Genet 105:127–138

    Article  PubMed  CAS  Google Scholar 

  • Dubouzet J, Shinoda GK, Murata N (1998) Interspecific hybridization of Allium gigantum Regel: production and early verification of putative hybrids. Theor Appl Genet 96:385–388

    Article  CAS  Google Scholar 

  • Franklin PG, Pearce RB, Roger LM (1985) Physiology of crop plants. Iowa State University Press, Ames, pp 201–242

    Google Scholar 

  • Garcia-Papi MA, Garcia-Martinez JL (1984) Endogenous plant growth substances content in young fruit of seeded and seedless Clementin Mandar in as related to fruit set and development. Sci Hortic 22:265–274

    Article  CAS  Google Scholar 

  • Gordillo LF, Jolley VD, Horrocks RD, Stevevs MR (2003) Interaction of BA, GA3, NAA, and surfactant on interspecific hybridization of Lycopersicon esculentum × L. chilense. Euphytica 131:15–23

    Article  CAS  Google Scholar 

  • Guillaumin J, Pierson J, Grassely C (2003) The susceptibility to Armillaria mellea of different Prunus species used as stone fruit rootstocks. Sci Hortic 46:43–54

    Article  Google Scholar 

  • Hirsch AM, Testolin R, Brown S, Chat J, Fortune D, Bureau JM, De Nay D (2001) Embryo rescue from interspecific crosses in the genus Actinidia (kiwifruit). Plant Cell Rep 20:508–516

    Article  CAS  Google Scholar 

  • Hormaza JI (1998) Early selection in cherry combining RAPDs with embryo culture. Sci Hortic 79:121–126

    Article  Google Scholar 

  • Infante R, Gonzalez J (2002) Early maturing peach embryo rescue and in vitro survival at different fruit growth stages. Acta Hortic 592:89–92

    Google Scholar 

  • Jose XC, Sherman WB (1994) Culture data and germination procedure affect success of nectarine ovule and embryo culture. Fruit Varieties J 48:173–175

    Google Scholar 

  • Kato J, Ishikawa R, Mii M (2001) Different genomic combinations in inter-section hybrids obtained from the crosses between Primula sieboldii (Section Cortusoids) and P. obconica (Section Obconicolisteri) by the embryo rescue technique. Theor Appl Genet 102:1129–1135

    Article  CAS  Google Scholar 

  • Kaushal R, Malaviya DR, Roy AK, Kumar B, Tiwari A (2005) Trifolium alexandrinum × T. resupinatum – interspecific hybrids developed through embryo rescue. Plant Cell Tissue Organ Cult 83:137–144

    Article  Google Scholar 

  • Kuden AB, Tantiver E, Gulen H (1999) Embryo rescue of peach hybrids. Acta Hortic 484:531–533

    Google Scholar 

  • Kumlehn J, Schieder O, Lörz H (1997) In vitro development of wheat (Triticum aestvum L.) from zygote to plant via ovule culture. Plant Cell Rep 16:663–667

    Article  CAS  Google Scholar 

  • Leopols AC, Nooden LO (1984) Hormonal regulatory system in plants. In: Scott TK (ed) Hormonal regulation of development II. Springer-verlag, Berlin, pp 10–11

    Google Scholar 

  • Liu HF, Chen XS, Duan CG, Yang HH, Feng BC (2004) Embryo rescue and identification of hybrids between sweet cherry and Chinese cherry (in Chinese with English abstract). Acta Hortic Sin 31:303–308

    Google Scholar 

  • Martínez-Gómez P, Dicenta F (2001) Mechanisms of dormancy in seeds of peach (Prunus persica (L.) Batsch) cv. GF305. Sci Hortic 91:51–58

    Article  Google Scholar 

  • Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue culture. Physiol Plant 15:473–497

    Article  CAS  Google Scholar 

  • Peixe A, Campos MD, Cavaleiro C, Barroso J, Pais MS (2000) Gamma-irradiated pollen induces the formation of 2n endosperm and abnormal embryo development in European plum (Prunus domestica L., cv. ‘Rainha Cláudia Verde’). Sci Hortic 4:267–278

    Article  Google Scholar 

  • Rode JC, Vaulx RD (1987) Obtention de plantes haploides de carotte (Daucus carota L.) issues de parthénogenèse induite in situ par du pollen irradié et culture in vitro des graines immatures. C R Acad Sci Paris (Série III) 305:225–229

    Google Scholar 

  • Roy AK, Malaviya DR, Kaushal P, Kumar B, Tiwari A (2004) Interspecific hybridization of Trifolium alexandrinum with T. constantinopolitanum using embryo rescue. Plant Cell Rep 22:705–710

    Article  PubMed  CAS  Google Scholar 

  • Rubio-Cabetas MJ, Socias R (1996) Fertilisation assessment and postzygotic development in several intra- and interspecific Prunus hybrids. Euphytica 90:325–330

    Article  Google Scholar 

  • Ryzhkov SD, Zhukov OS, Kharitonova EN (1975) Effect of an electromagnetic field on the fertilizing capacity of the pollen in the distant hybridization of stone fruits. Michurina 22:62–64

    Google Scholar 

  • Ryzhkov SD, Zhukov OS, Osipova LV (1979) Gentic effect of using electrically separated and irradiated pollen in the distant hybridization of Prunus besseyi and apricot. Subtropich Kult 3:89–90

    Google Scholar 

  • Ryzhkov SD, Ostapenko VI (1974) The physical barrier of sexual incompatibility and the mechanism causing it in the distant hybridization of fruit crops. Michurina 15:61–67

    Google Scholar 

  • Sestili S, Ficcadenti N (1996) Irradiated pollen for haploid production. In: Jain S et al (eds) In vitro haploid production in higher plants, vol I. Kluwer Academic Publishers, Dordrecht, pp 263–274

    Google Scholar 

  • Sharma DR, Kaur R, Kumar K (1996) Embryo rescue in plants – a review. Euphytica 89:325–337

    Google Scholar 

  • Sosinski B, Gannavarapu M, Hager LD, Beck LE, King GJ, Ryder CD, Rajapakse S, Baird WV, Ballard RE, Abbott AG (2000) Characterization of microsatellite markers in peach [Prunus persica (L.) Batsch]. Theor Appl Genet 101:421–428

    Article  CAS  Google Scholar 

  • Streiff R, Labbe T, Bacilieri R, Steinkellner H, Glossl J, Kremer A (1998) Within-population genetic structure in Quercus robur L., and Quercus petraea (Matt.) Liebl. assessed with isozymes and microsatellites. Mol Ecol 7:317–328

    Article  Google Scholar 

  • Struss D, Boritzki M, Glozer K (2001) Detection of genetic diversity among populations of sweet cherry (Prunus avium L.) by AFLPs. J Hort Sci Biotech 76:362–367

    CAS  Google Scholar 

  • Suzuki H, Ishikawa T, Hiura I (1988) Interspecific hybridization between sweet cherry (P.avium L.) and P. apetala Fr. Et Sav. Var. Pilosa. J Yamagata Agric For Soc 45:9–18

    Google Scholar 

  • Tukey HB (1933) Artificial culture of sweet cherry embryos. J Hered 24:7–12

    Google Scholar 

  • Valk PVD, Vries SD, Verstappen F (1991) Pre- and post-fertilization barriers to backcrossing the interspecific hybrid between Allium fistulosum L. and A. cepa L. with A. cepa. Euphytica 53(3):201–209

    Article  Google Scholar 

  • Van Creij MGM, Kerckhoffs DMFJ, Van Tuyl JM (1997) Interspecific cross in the genus Tulipa L. identificaition of pre-fertilization barriers. Sex Plant Reprod 10:116–123

    Article  Google Scholar 

  • Viorica B, Antonia I, Gabriela C (1999) Studies on immature embryos in in vitro culture of interspecifc hybrids from the genus Prunus. Acta Hortic 484:17–19

    Google Scholar 

  • Wang YZ, Zhang DP, Yang L, Yan AL, Shi H (2000) Study on the techniques of in vitro embryo culture with immature embryo of apricot (in Chinese with English abstract). J Agric Biotechnol 8:389–391

    Google Scholar 

  • Yang HH, Chen XS, Feng BC, Liu HF, Zheng Z (2004) Creating new germplasm by distant hybridization in stone fruit: II – Embryo rescue and hybrid identification between plum and apricot. Agric Sci China 37:1203–1307

    Google Scholar 

  • Zhang YX, Lespinasse Y (1991) Pollination with gamma-irradiated pollen and development of fruits, seeds and parthenogenic plants in apple. Euphytica 54:101–109

    Article  Google Scholar 

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Acknowledgments

We thank Mr. Xiujia Mu for providing excellent technical assistance for this study. Financial support by the National Natural Science Foundation of China (NSFC) is greatly appreciated.

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Correspondence to Xuesen Chen.

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Liu, W., Chen, X., Liu, G. et al. Interspecific hybridization of Prunus persica with P. armeniaca and P. salicina using embryo rescue. Plant Cell Tiss Organ Cult 88, 289–299 (2007). https://doi.org/10.1007/s11240-007-9201-z

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