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Interspecific hybridization of Prunus persica with P. armeniaca and P. salicina using embryo rescue

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Abstract

Embryo rescue technique was used successfully to produce interspecific hybrids by crossing peach (P. persica) as a female parent with apricot (P. armeniaca) and plum (P. salicica). In those crosses that had ‘Yuhualu’ or ‘Zhonghuashoutao’ as female parents, hybrid embryos aborted from the 7th or 8th week after pollination mainly due to post-pollination incompatibility. An embryo rescue protocol was established to rescue such embryos and recover hybrid plants. Modified half-strength MS medium containing 4 mg l−1 6-BA and 0.5 mg l−1 IBA produced up to 90% germination in the embryos. Modified MS medium with 1.0 mg l−1 6-BA and 1.0 mg l−1 IBA gave the highest bud induction and multiplication whereas modified MS medium containing 0.5 mg l−1 IAA and 0.2 mg l−1 NAA gave the best rooting percentage. All the hybrids obtained using this embryo rescue technique were verified using simple sequence repeat (SSR) markers. A series of pollen treatments were carried out to partially overcome pre-pollination incompatibility, and it was found accidentally that pollen treatment with electrostatic field not only improved pollen germination but also increased the multiplication coefficient of embryo-induced shoots.

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Abbreviations

IAA:

indole-3-acetic acid

IBA:

indole-3-butyric acid

NAA:

α-naphthaleneacetic acid

6-BA:

6-benzyladenine

MS:

Murashige and Skoog (1962) medium

PF:

embryo length/seed length

SSR:

simple sequence repeat

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Acknowledgments

We thank Mr. Xiujia Mu for providing excellent technical assistance for this study. Financial support by the National Natural Science Foundation of China (NSFC) is greatly appreciated.

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Authors and Affiliations

  1. Fruit Biology Laboratory, College of Horticulture Science and Engineering, Shandong Agricultural University, Shandong, 271018, PR China

    Wen Liu, Xuesen Chen, Qing Liang, Tianming He & Jianrong Feng

  2. College of Food Science and Engineering, Shandong Agricultural University, Shandong, 271018, PR China

    Guanjun Liu

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  1. Wen Liu
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  2. Xuesen Chen
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  3. Guanjun Liu
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  4. Qing Liang
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  5. Tianming He
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Correspondence to Xuesen Chen.

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Liu, W., Chen, X., Liu, G. et al. Interspecific hybridization of Prunus persica with P. armeniaca and P. salicina using embryo rescue. Plant Cell Tiss Organ Cult 88, 289–299 (2007). https://doi.org/10.1007/s11240-007-9201-z

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  • DOI: https://doi.org/10.1007/s11240-007-9201-z

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