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A two-step procedure for in vitro multiplication of cloudberry (Rubus chamaemorus L.) shoots using bioreactor

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Abstract

Cultures of three cloudberry (Rubus chamaemorus L.) clones collected from natural stands in Newfoundland and Labrador, Canada were established in vitro on a modified cranberry (Vaccinium macrocarpon Ait.) tissue culture medium containing 8.9 μM 6-benzylaminopurine (BAP). Clones were compared for in vitro shoot proliferation on gelled medium supplemented with varying levels of BAP and thidiazuron (TDZ). Addition of 5.8 μM gibberellic acid (GA3) in 8.9 μM BAP-contained medium improved shoot proliferation. TDZ supported rapid shoot proliferation at low concentration (1.1 μM) but induced 20–30% hyperhydricity in a plastic airlift bioreactor system containing liquid medium. Bioreactor-multiplied hyperhydric shoots were transferred to gelled medium containing 8.9 μM BAP and 5.8 μM GA3 and produced normal shoots within 4 weeks of culture. Genotypes differed significantly with respect to multiplication rate with ‘C1’ producing the most shoots per explant. Proliferated shoots were rooted on a potting medium with 65–75% of survivability of rooted plants. Present results suggested the possibility of large-scale multiplication of cloudberry shoots in bioreactors.

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Abbreviations

BAP:

6-Benzylaminopurine

IBA:

Indole-3-butyric acid

GA3 :

Gibberellic acid

PPF:

Photosynthetic photon flux

TDZ:

Thidiazuron

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Acknowledgements

Atlantic Cool Climate Crop Research Centre Contribution no. 182. The author wishes to thank Sarah Devine, Glen Chubbs, Darryl Martin, Shawn Foley, and Erica Stone for their excellent technical help.

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  1. Atlantic Cool Climate Crop Research Centre, Agriculture and Agri-Food Canada, 308 Brookfield Road, P.O. 39088, St. John’s, Newfoundland and Labrador, Canada, A1E 5Y7

    Samir C. Debnath

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  1. Samir C. Debnath
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Correspondence to Samir C. Debnath.

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Debnath, S.C. A two-step procedure for in vitro multiplication of cloudberry (Rubus chamaemorus L.) shoots using bioreactor. Plant Cell Tiss Organ Cult 88, 185–191 (2007). https://doi.org/10.1007/s11240-006-9188-x

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  • DOI: https://doi.org/10.1007/s11240-006-9188-x

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