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In vitro somatic embryogenesis and plant regeneration in Zantedeschia hybrids

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Abstract

A method for regeneration of plants from tuber explants of a Zantedeschia hybrid via somatic embryogenesis was developed. In vitro cultures were initiated starting from both anthers and tubers. Somatic embryogenesis was only achieved from tuber explants. 6-Benzyladenine (BA) at 0.6 or 2 mg l−1 in combination with 2 mg l−1 α-naphthaleneacetic acid (NAA) yielded the highest number of embryos per explant. The somatic embryos converted into plantlets on Murashige and Skoog basal medium supplemented with vitamins, micro- and macronutrients, 1 mg l−1 6-τ-τ-(dimethylallylamino)-purine (2iP), 3% sucrose and 0.7% agar. This is the first report on induction of somatic embryogenesis in Zantedeschia.

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Fig. 1

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Abbreviations

BA:

6-benzyladenine

2,4-D:

2,4-dichlorophenoxyacetic acid

2iP:

6-τ-τ-(dimethylallylamino)-purine

MS:

Murashige and Skoog basal medium

NAA:

α-naphthaleneacetic acid

TDZ:

Thidiazuron

References

  • Chen F, Kuehnle A (1999) Micropropagation of Spathiphyllum cv. Sensation from in vitro etiolated internode explants. In: XVI International Botanical Congress, St Louis, Book of abstracts, nr 2383

  • Funnell K (1993). Zantedeschia. In: De Hertogh A, Le Nard M (eds) The physiology of flower bulbs. Elsevier, Amsterdam

    Google Scholar 

  • Gomez L, Valverde R, Arias O, Thorpe T (1992). Regeneration of Xanthosoma sagittifolium by somatic embryogenesis. Agron Costarric 16:219–223

    Google Scholar 

  • Hamidah M, Karim A, Debergh P (1997). Somatic embryogenesis and plant regeneration in Anthurium scherzerianum. PCTOC 48:189–193

    Article  Google Scholar 

  • Matsumoto T, Webb D, Kuehnle A (1996). Histology and origin of somatic embryos derived rom Anthurium andreanum Linden ex Andre lamina. J Am Soc Hort Sci 121:404–407

    Google Scholar 

  • Murashige T, Skoog F (1962). A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol Plant 15:473–497

    Article  CAS  Google Scholar 

  • Werbrouck S, Eeckhaut T, Debergh P (2000). Induction and conversion of somatic embryogenesis on the anther filament of Spathiphyllum Schott. Acta Hortic 520:263–269

    Google Scholar 

  • Wu A, Zhu B, Li Q (1996). Studies on somatic embryogenesis of Pinellia pedatisecta Schott. Acta Agron. Sin 22:66–71

    Google Scholar 

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Authors and Affiliations

  1. Plant Unit – Applied Genetics and Breeding, Institute for Agricultural and Fisheries Research, Caritasstraat 21, 9090, Melle, Belgium

    B. Duquenne, T. Eeckhaut & J. Van Huylenbroeck

  2. Department of Biotechnology, Hogeschool Gent, Voskenslaan 270, 9000, Gent, Belgium

    B. Duquenne & S. Werbrouck

Authors
  1. B. Duquenne
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  2. T. Eeckhaut
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  3. S. Werbrouck
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  4. J. Van Huylenbroeck
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Correspondence to B. Duquenne.

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Duquenne, B., Eeckhaut, T., Werbrouck, S. et al. In vitro somatic embryogenesis and plant regeneration in Zantedeschia hybrids. Plant Cell Tiss Organ Cult 87, 329–331 (2006). https://doi.org/10.1007/s11240-006-9161-8

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  • DOI: https://doi.org/10.1007/s11240-006-9161-8

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