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Induction of embryogenic tissue and maturation of somatic embryos in Pinus brutia TEN

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Abstract

Embryogenic tissues (ET) of Turkish red pine (Pinus brutia TEN) were initiated from immature precotyledonary zygotic embryos sampled from 15 different plus trees. Seven collections were made weekly from June 10 to July 22, 2003. DCR basal medium supplemented with 13.6 μM 2,4–dichlorophenoxyacetic acid (2,4-D) and 2.2 μM benzylaminopurine (BAP) was used for initiation and maintenance of ET. Overall initiation frequency of ET in the study was 11.6%, initiation rates ranging between 4.7% and 24.1% per tree. Out of 12,940 explants tested, 3.4% were converted into established cell lines (ECL) following five subcultures. Of the maturation treatments tested, 80 μM ABA, sucrose (3%) and maltose (3% and 6%), and 3.75% PEG combined with 1% gellan gum were the most suitable combination for somatic embryo maturation.

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Abbreviations

ABA:

Abscisic acid

BAP:

Benzylaminopurine

2,4-D :

Dichlorophenoxyacetic acid

ECL:

Established cell line

ET:

Embryogenic tissue

INFREQ:

Initiation frequency

PEG:

Polyethyleneglycol

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Acknowledgements

This research was funded by TUBİTAK (The Scientific and Technological Council of Turkey) Project No: TOGTAG-2634/3076, and METU (Middle East Technical University, Ankara) Project No: BAP-08-11-DPT2002K120510. The authors are grateful to Southwestern Anatolia Forest Research Institute (Antalya) and TEMA Foundation for their support in collecting immature seed cones and providing infrastructure, respectively.

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  1. Tolga Yildirim

    Present address: Department of Biology, Akdeniz University, 07058, Antalya, Turkey

Authors and Affiliations

  1. Department of Biological Sciences, Middle East Technical University, 06531, Ankara, Turkey

    Tolga Yildirim & Zeki Kaya

  2. Department of Biology, Akdeniz University, 07058, Antalya, Turkey

    Kani Işik

Authors
  1. Tolga Yildirim
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  2. Zeki Kaya
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  3. Kani Işik
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Correspondence to Zeki Kaya.

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Yildirim, T., Kaya, Z. & Işik, K. Induction of embryogenic tissue and maturation of somatic embryos in Pinus brutia TEN. Plant Cell Tiss Organ Cult 87, 67–76 (2006). https://doi.org/10.1007/s11240-006-9137-8

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  • DOI: https://doi.org/10.1007/s11240-006-9137-8

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