Skip to main content

Advertisement

SpringerLink
Log in

Salt and paraquat stress tolerance results from co-expression of the Suaeda salsa glutathione S-transferase and catalase in transgenic rice

  • Original paper
  • Published:
Plant Cell, Tissue and Organ Culture Aims and scope Submit manuscript

Abstract

GST (Glutathione S-transferase, EC 2.5.1.18) and CAT (Catalase, EC 1.11.1.6) play important roles in oxidative stress resistance. In this study, we transferred both GST and CAT1 of Suaeda salsa into rice (Oryza sativa cv. Zhonghua No.11) by Agrobacterium tumefaciens-mediated transformation under the control of cauliflower mosaic virus (CaMV) 35S promoter, and investigated whether co-expressing the GST and CAT1 in transgenic rice could reduce oxidative damage. Salt and paraquat stresses were applied. The data showed that co-expression of the GST and CAT1 resulted in greater increase of CAT and SOD (Superoxide Dismutase, EC 1.15.1.1) activity in the transgenics compared to non-transgenics following both stress imposition. Whereas the significant increase of GST activity in transgenics only occurred in paraquat stressed plants. While the generation of H2O2, Malon dialdehyde and plasma membrane relative electrolyte leakage decreased in the transgenics than in non-transgenics under the same conditions. Moreover, the transgenic rice seedlings showed markedly enhanced tolerance to salt stress compared with non-transgenics upon 200 mM NaCl treatment in greenhouse. The enhancement of the active oxygen-scavenging system that led to increased oxidative stress protection in GST  + CAT1-transgenic rice plants could result not only from increased GST and CAT activity but also from the combined increase in SOD activity.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

Abbreviations

CDNB-1:

Chloro-2,4-dinitrobenzene

DAB-3:

3-Diaminobenzidine

MV:

Methyl viologen

PEL:

Plasma membrane relative electrolyte leakage

References

  • Alscher RG, Erturk N, Heath LS (2002) Role of superoxide dismutases (SODs) in controlling oxidative stress in plants. J Exp Bot 53:1331–1341

    Article  PubMed  CAS  Google Scholar 

  • Anderson M, Chen Z, Klessig DF (1998) Possible involvement of lipid peroxidation in salicylic acid-mediated induction of PR-1 gene expression. Phytochemistry 47:555–566

    Article  CAS  Google Scholar 

  • Bowler C, van Montagu M, Inze D (1992) Superoxide dismutase and stress tolerance. Annu Rev Plant Physiol Plant Mol Biol 43:83–116

    Article  CAS  Google Scholar 

  • Bradford MM (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Ana1 Biochem 72:248–254

    Article  CAS  Google Scholar 

  • Chen W, Chao G, Singh KB (1996) The promoter of a H2O2-inducible Arabidopsis glutathione S-transferase gene contains closely linked OBF- and OBP1-binding sites. Plant J 10:955–966

    Article  PubMed  CAS  Google Scholar 

  • Dixon DP, Davis BG, Edwards R (2002) Functional divergence in the glutathione transferase superfamily in plants—identification of two classes with putative functions in redox homeostasis in Arabidopsis thaliana. J Biol Chem 277:30859–30869

    Article  PubMed  CAS  Google Scholar 

  • Dorey S, Baillieul F, Saindrenan P, Fritig B, Kauffmann S (1998) Tobacco class I and II catalases are differentially expressed during elicitor-induced hypersensitive cell death and localized acquired resistance. Mol Plant-Microbe Interact 11:1102–1109

    Article  CAS  Google Scholar 

  • Gao XH, Ren ZH, Zhao YX, Zhang H (2003) Overexpression of SOD2 increases salt tolerance of Arabidopsis. Plant Physiol 133:1873–1881

    Article  PubMed  CAS  Google Scholar 

  • Gupta AS, Webb RP, Holaday AS, Allen RD (1993) Overexpression of superoxide dismutase protects plants from oxidative stress. Induction of ascorbate peroxidase in superoxide dismutase-overexpressing plants. Plant Physiol 103:1067–1073

    PubMed  Google Scholar 

  • Heath RL, Packer L (1968) Photoperoxidation in isolated chloroplasts I. Kinetics and stoichiometry of fatty acid peroxidation. Arch Biochem Biophysiol 125:189–198

    Article  CAS  Google Scholar 

  • Jepson I, Holt DC, Roussel V, Wright SY, Greenland AJ (1997) Transgenic plant analysis as a tool for the study of maize glutathione S-transferases. In: Hatzios KK (ed) Regulation of enzymatic systems detoxifying xenobiotics in Plants. Kluwer Academic Publishers, Dordrecht, Netherlands, pp 313–323

    Google Scholar 

  • Kampranis SC, Damianova R, Atallah M, Toby G, Kondi G, Tsichlisi PN, Makris AM (2000) A novel plant glutathione S-transferase/peroxidase suppresses Bax lethality in yeast. J Biol Chem 275:29207–29216

    Article  PubMed  CAS  Google Scholar 

  • Lin ZF, Li SS, Lin GZ (1988) The accumulation of hydrogen peroxide in senescencing leaves and chloroplasts in relation to lipid peroxidation. Acta Phytophysiol Sin 14:16–22

    CAS  Google Scholar 

  • Ma CL, Wang PP, Cao ZY, Zhao YX, Zhang H (2003) Cloning and differencial gene expression of catalases in Suaeda salsa in response to salt stress. Acta Botanica Sin 45:93–97

    CAS  Google Scholar 

  • Mauch F, Dudlar R (1993) Differential induction of distinct glutathione-S-transferases of wheat by xenobiotics and by pathogen attack. Plant Physiol 102:1193–1201

    Article  PubMed  CAS  Google Scholar 

  • Miyagawa Y, Tamoi M, Shigeoka S (2000) Evaluation of the defense system in chloroplasts to photooxidative stress caused by paraquat using transgenic tobacco plants expressing catalase from Escherichia coli. Plant Cell Physiol 41:311–320

    PubMed  CAS  Google Scholar 

  • Moran JF, Becana M, Iturbe-Ormaetxe I, Frechilla S, Klucas RV, Aparicio-Tejo P (1994) Drought induces oxidative stress in pea plants. Planta 194:346–352

    Article  CAS  Google Scholar 

  • Payton P, Allen RD, Trolinder N, Holaday AS (1997) Overexpression of chloroplast-targeted Mn superoxide dismutase in cotton (Gossypium hirsutum L., cv. Coker 312) does not alter the reduction of photosynthesis after short exposures to low temperature and high light intensity. Photosyn Res 52:233–244

    Article  CAS  Google Scholar 

  • Price AH, Taylorail A, Ripley SJ, Griffiths A, Ttewavas AJ, Knight MR (1994) Oxidative signals in tobacco increase cytosolic calcium. Plant Cell 6:1301–1310

    Article  PubMed  CAS  Google Scholar 

  • Polidoros AN, Scandalios JG (1999) Role of hydrogen peroxide and different classes of antioxidants in the regulation of catalase and glutathione S-transferase gene expression in maize (Zea mays L.). Physiol Plant 106:112–120

    Article  CAS  Google Scholar 

  • Polidoros AN, Mylona PV, Scandalios JG (2001) Transgenic tobacco plants expressing the maize Cat2 gene have altered catalase levels that affect plant–pathogen interactions and resistance to oxidative stress. Transgenic Res 10:555–569

    Article  PubMed  CAS  Google Scholar 

  • Rao MV, Paliyath G, Ormrod DP (1997) Influence of salicylic acid on H2O2 production, oxidative stress, and H2O2-metabolizing enzymes. Plant Physiol 115:137–149

    Article  PubMed  CAS  Google Scholar 

  • Rizhsky L, Hallak-Herr E, Van Breusegem F, Rachmilevitch S, Barr JE, Rodermel S, Inze D, Mittler R (2002) Double antisense plants lacking ascorbate peroxidase and catalase are less sensitive to oxidative stress than single antisense plants lacking ascorbate peroxidase or catalase. Plant J 32:329–342

    Article  PubMed  CAS  Google Scholar 

  • Roxas VP, Smith RK, Allen ER, Allen RD (1997) Overexpression of glutathione S-transferase/glutathione peroxidase enhances the growth of transgenic tobacco seedlings during stress. Nat Biotechnol 15:988–991

    Article  PubMed  CAS  Google Scholar 

  • Shigeoka S, Ishikawa T, Tamoi M, Miyagawa Y, Takeda T, Yabuta Y, Yoshimura K (2002) Regulation and function of ascorbate peroxidase isoenzymes. J Exp Bot 53:1305–1319

    Article  PubMed  CAS  Google Scholar 

  • Talarczyk A, Krzymowska M, Borucki W, Hennig J (2002) Effect of yeast CAT1 gene expression on response of tobacco plants to Tobacco Mosaic Virus infection. Plant Physiol 129:1032–1044

    Article  PubMed  CAS  Google Scholar 

  • Wang LP, Qi YC, Zhao YX, Zhang H (2002) Cloning and sequencing of GST gene of Suaeda salsa and its expression characteristics. J Plant Physiol Mol Biol 28:133–136

    CAS  Google Scholar 

  • Willekens H, Chamnongpol S, Davey M, Schraudner M, Langebartels C, Van Montagu M, Inze D, Van Camp W (1997) Catalase is a sink for H2O2 and is indispensable for stress defence in C3 plants. EMBO J 16:4806–4816

    Article  PubMed  CAS  Google Scholar 

  • Yabuta Y, Maruta T, Yoshimura K, Ishikawa T, Shigeoka S (2004) Two distinct redox signaling pathways for cytosolic APX induction under photooxidative stress. Plant Cell Physiol 45:1586–1594

    Article  PubMed  CAS  Google Scholar 

  • Yu T, Li YS, Chen XF, Hu J, Chang X, Zhu YG (2003) Transgenic tobacco plants overexpressing cotton glutathione S-transferase (GST) show enhanced resistance to methyl viologen. J Plant Physiol 160:1305–1311

    Article  PubMed  CAS  Google Scholar 

  • Zelitch I (1992) Factors affecting expression of enhanced catalase activity in a tobacco mutant with O2-resistant photosynthesis. Plant Physiol 98:1330–1335

    Article  PubMed  CAS  Google Scholar 

  • Zhao FY, Guo SL, Zhang H, Zhao YX (2006) Expression of yeast SOD2 in transgenic rice results in increased salt tolerance. Plant Sci 170:216–224

    Article  CAS  Google Scholar 

  • Zhao KF (1993) Salt tolerance physiology of plant. Science and Technology Press, Beijing, China, pp 221–234

    Google Scholar 

Download references

Acknowledgements

This work is supported financially by the National High Technology Research and Development Program of China (863 Program No.2002AA629080) and National Key Fundamental Research Program of China (973 Program No.2006CB100104).

Author information

Authors and Affiliations

  1. Life Science College, Shandong University of Technology, Zibo, 255049, Shandong Province, P.R. China

    Fengyun Zhao

  2. Key Laboratory of Plant Stress, Life Science College, Shandong Normal University, Jinan, 250014, Shandong Province, P.R. China

    Hui Zhang

Authors
  1. Fengyun Zhao
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Hui Zhang
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Hui Zhang.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Zhao, F., Zhang, H. Salt and paraquat stress tolerance results from co-expression of the Suaeda salsa glutathione S-transferase and catalase in transgenic rice. Plant Cell Tiss Organ Cult 86, 349–358 (2006). https://doi.org/10.1007/s11240-006-9133-z

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s11240-006-9133-z

Keywords

Search

Navigation