Abstract
We tested the organogenetic capacity of floral buds of Mammillaria albicoma Böed. (Cactaceae). Buds were incubated on solid MS medium supplemented with 0.1 mg l−1 α-naphthaleneacetic acid (NAA) and 5.0 mg l−1 6-benzylaminopurine (BA). Callus growth was observed from the cut explant base and from within the perianth. These calli during subsequent subcultures to the same medium gave rise to adventitious shoots. Shoots formed also directly from the perianth, as confirmed by observations in the light microscope and scanning electron microscope (SEM). On transfer to a fresh medium, the shoots produced proliferating cultures. This is the first report of regeneration of cactus shoots from floral explants.
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Abbreviations
- BA:
-
6-benzylaminopurine
- MS:
-
Murashige and Skoog
- NAA:
-
α-naphthaleneacetic acid
- PPFD:
-
photosynthetic photon flux density
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We thank Dr. David Giblin for linguistic advice.
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Wyka, T.P., Hamerska, M. & Wróblewska, M. Organogenesis of vegetative shoots from in vitro cultured flower buds of Mammillaria albicoma (Cactaceae) . Plant Cell Tiss Organ Cult 87, 27–32 (2006). https://doi.org/10.1007/s11240-006-9128-9
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DOI: https://doi.org/10.1007/s11240-006-9128-9