A new protocol for the preparation and sterilization of media with sodium hypochlorite was tested for in vitro culturing of pineapple shoots. Total active chlorine concentrations equal to or higher than 0.0003%, as well as other asepsis procedures, provided complete sterilization of the medium. At a total active chlorine concentration of 0.0003%, pineapple shoots more than doubled both their biomass and the number of new shoots.
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We are indebted to FENORTE/TECNORTE and FAPERJ (Brazil) for funding this research work.
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Teixeira, S.L., Ribeiro, J.M. & Teixeira, M.T. Influence of NaClO on nutrient medium sterilization and on pineapple (Ananas comosus cv Smooth cayenne) behavior. Plant Cell Tiss Organ Cult 86, 375–378 (2006). https://doi.org/10.1007/s11240-006-9121-3
- Biomass production
- Chemical sterilization
- Medium contamination