Skip to main content
Log in

Anther culture and haploid plant regeneration in purple coneflower (Echinacea purpurea L.)

  • Original paper
  • Published:
Plant Cell, Tissue and Organ Culture Aims and scope Submit manuscript

Abstract

Anthers containing microspores at the uninucleate stage were excised from capitula of field grown plants of purple coneflower, Echinacea purpurea, and cultured on medium conducive to callus growth. In callus induction cultures, N6 basal medium was more effective than Murashige and Skoog (MS), and a combination of benzyladenine (BA) at 2.22 µM with naphthaleneacetic acid (NAA) at 0.054 µM was more effective than 2,4-dichlorophenoxyacetic acid (2,4-D) alone at 4.52, 9.05 and 13.57 µM. Callus induction rate as high as 85.8% was achieved, and no statistically significant differences were found among cultures with various densities of 20, 40, 60, 80 and 100 anthers per bottle each filled with 40-ml medium. Shoots were regenerated from calluses on MS medium containing 2.22 µM BA and various concentrations of NAA, with the highest regeneration rate of 95.24% obtained when 0.27 µM NAA was applied. Although a large portion of the␣regenerated shoots had prominent symptom of vitrification, some normal shoots could be easily rooted on MS medium containing 0.054 µM NAA. Thirty regenerated plants were randomly selected and 19 of them were confirmed to be haploid by observation of chromosome number of root-tip cells.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Subscribe and save

Springer+ Basic
EUR 32.99 /Month
  • Get 10 units per month
  • Download Article/Chapter or Ebook
  • 1 Unit = 1 Article or 1 Chapter
  • Cancel anytime
Subscribe now

Buy Now

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Similar content being viewed by others

Abbreviations

BA:

benzyladenine

NAA:

naphthaleneacetic acid

2,4-D:

2,4-dichlorophenoxyacetic acid

MS:

Murashige and Skoog (1962)

References

  • Barrett B (2003) Medicinal properties of Echinacea: a critical review. Phytomedicine 10:66–86

    Article  PubMed  CAS  Google Scholar 

  • Bauer R (1999) Chemistry, analysis and immunological investigations of Echinacea phytopharmaceuticals. In: Wagner H (ed) Immunomodulatory agents from plants. Birkhauser Verlag. pp 41–88

  • Bhatti S, Aziz AN, Sauve R (2001) Anther culture response of Echinacea cultivars. 23rd Annual University-Wide Research Symposium, Tennessee State University, Nashville, TN, March 20–21, p 39

  • Borrino EM, Powell W (1988) Stomata guard cell length as an indicator of ploidy in microspore-derived plants of barley. Genome 30:158–160

    Article  Google Scholar 

  • Choffe KL, Victor JMR, Murch SJ, Saxena PK (2000) In vitro regeneration of Echinacea L.: direct somatic embryogenesis and indirect shoot organogensis in petiole culture. In Vitro Cell Dev Biol Plant 36:30–36

    Article  CAS  Google Scholar 

  • Chu CC, Wang CC, Su CS (1975) Establishment of an efficient medium for anther culture of rice through comparative experiments on the nitrogen sources. Sci Sin 18:659–668

    Google Scholar 

  • Gu XF, Yang AF, Meng H, Zhang JR (2005) In vitro induction of tetraploid plants from diploid Zizyphus jujuba Mill. cv. Zhanhua. Plant Cell Rep 24:671–676

    Article  PubMed  CAS  Google Scholar 

  • Harbage JF (2001) Micropropagation of Echinacea angustifolia, E. pallida and E. purpurea from stem and seed explants. Hortscience 36:360–364

    CAS  Google Scholar 

  • Hobbs CR (1994) Echinacea – a literature review. Herbalgram 30:33–49

    Google Scholar 

  • Kao TH, McCubbin AG (1996) How flowering plants discriminate between self and non-self pollen to prevent␣inbreeding. Proc Natl Acad Sci USA 93:12059–12065

    Article  PubMed  CAS  Google Scholar 

  • Koroch A, Juliani HR, Kapteyn J, Simon JE (2002) In vitro regeneration of Echinacea purpurea from leaf explants. Plant Cell Tiss Organ Cult 69:79–83

    Article  CAS  Google Scholar 

  • Kuhlman U, Foroughi B (1989) Production of doubled haploid lines in frequencies sufficient for barley breeding programs. Plant Cell Rep 8:78–81

    Article  Google Scholar 

  • Lakshmanan P, Danesh M, Taji A (2002) Production of four commercially cultivated Echinacea species by different methods of in vitro regeneration. J Horticult Sci Biotech 77:158–163

    Google Scholar 

  • Lee SY, Kim HS, Kwon TO (2004) Variation in anther culture response and fertility of backcrossed hybrids between indica and japonica rice (Oryza sativa). Plant Cell Tiss Organ Cult 79:25–30

    Article  Google Scholar 

  • Li TCS (1998) Echinacea: cultivation and medicinal value. HortTechnology 8:122–129

    Google Scholar 

  • McKeown KA (1999) A review of taxonomy of the genus Echinacea. In: Janick J (eds) Perspectives on new crops and new uses, Purdue University, West Lafayette, pp 482–490

    Google Scholar 

  • Mechanda SM, Baum BR (2003) Direct shoot regeneration from leaf segments of mature plants of Echinacea purpurea (L.) Moench. In Vitro Cell Dev Biol Plant 39:505–509

    Article  Google Scholar 

  • Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol Plant 15:473–497

    Article  CAS  Google Scholar 

  • Portela de Carvalho JFR, Roberto de Carvalho C, Otoni WC (2005) In vitro induction of polyploidy in annatto (Bixa orellana). Plant Cell Tiss Organ Cult 80:69–75

    Article  CAS  Google Scholar 

  • Qu L, Wang X, Hood E, Wang M, Scalzo R (2004) Chromosome karyotypes of Echinacea angustifolia var. angustifolia and E. purpurea. HortScience 39:368–370

    PubMed  Google Scholar 

  • Sauve RJ, Mmbaga MT, Zhou S (2004) In vitro regeneration of the Tennessee coneflower (Echinacea tennesseensis). In Vitro Cell Dev Biol Plant 40:325–328

    Article  CAS  Google Scholar 

  • Sejdler LK, Dabrowska J (1996) Studies on the biology of flowering and fruiting of purple coneflower (Echinacea purpurea Moench). Pt. 1. Biology of flowering and fruiting. Herba Polonica 42:83–87

    Google Scholar 

  • Silva NF, Goring DR (2001) Mechanisms of self-incompatibility in flowering plants. Cell Mol Life Sci 58:1988–2007

    Article  PubMed  CAS  Google Scholar 

  • Thao NTP, Ureshino K, Miyajima I, Ozaki Y, Okubo H (2003) Induction of tetraploids in ornamental Alocasia through colchicine and oryzalin treatments. Plant Cell Tiss Organ Cult 72:19–25

    Article  CAS  Google Scholar 

  • Ziauddin A, Marsolais A, Simion E (1992) Improved plant regeneration from wheat anther and barley microspore culture using phenylacetic acid (PAA). Plant Cell Rep 11:489–498

    Article  CAS  Google Scholar 

  • Zobayed SMA, Saxena PK (2003) In vitro regeneration of Echinacea purpurea L.: enhancement of somatic embryogenesis by indole butyric acid and dark pre-incubation. In Vitro Cell Dev Biol Plant 39:605–612

    Article  CAS  Google Scholar 

Download references

Acknowledgements

This research was supported by a grand (2004Z3-E5021) from the Science and Technology Bureau of the Guangzhou Municipal Government.

Author information

Authors and Affiliations

Authors

Corresponding author

Correspondence to Yue-Sheng Yang.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Zhao, FC., Nilanthi, D., Yang, YS. et al. Anther culture and haploid plant regeneration in purple coneflower (Echinacea purpurea L.). Plant Cell Tiss Organ Cult 86, 55–62 (2006). https://doi.org/10.1007/s11240-006-9096-0

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s11240-006-9096-0

Keywords

Navigation