Abstract
Shoots of rose (hybrid tea) cv. “First Prize” were induced to flower in vitro on Murashige and Skoog (MS) medium containing various sucrose concentrations (15, 30 or 45 g l−1) and different phytohormone combinations of different cytokinins [N6-benzyladenine (BA); thidiazuron (TDZ) and zeatin] with α-naphthaleneacetic acid (NAA). Results indicate that sucrose is the key factor in floral morphogenesis while cytokinin increases the flowering percentage and helps the normal development of floral buds. From the three cytokinins that were used, BA and zeatin were considered to be more suitable as inductive flowering agents than TDZ. Reduced inorganic and organic salt concentration in MS media had a positive effect on in vitro flowering. The morphology of shoots bearing floral buds varied with different cytokinin treatments. The highest percentage (45%) of flowering was obtained on MS medium supplemented with 3.0 mg l−1 BA, 0.1 mg l−1 NAA and 30 g l−1 sucrose.
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Abbreviations
- BA:
-
N6-benzyladenine
- TDZ:
-
Thidiazuron
- NAA:
-
α-Naphthaleneacetic acid
- MS:
-
Murashige and Skoog
- PGR:
-
Plant growth regulator
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Vu, N.H., Anh, P.H. & Nhut, D.T. The role of sucrose and different cytokinins in the in vitro floral morphogenesis of rose (hybrid tea) cv. “First Prize”. Plant Cell Tiss Organ Cult 87, 315–320 (2006). https://doi.org/10.1007/s11240-006-9089-z
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DOI: https://doi.org/10.1007/s11240-006-9089-z