Embryo rescue and plant regeneration in vitro of selfed chickpea (Cicer arietinum L.) and its wild annual relatives
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The main constraint to the transfer of desired traits into cultivated chickpea from wild Cicer relatives is the presence of post-zygotic barriers which result in abortion of the immature embryo following interspecific hybridisation. Rescue of hybrid embryos in vitro and regeneration of hybrid plantlets could allow chickpea breeders to transfer desirable traits from wild relatives of chickpea. The development of embryo rescue techniques using selfed chickpea and selfed wild relatives is being used as a first step to protocols for wide hybrids. Optical microscopy studies of embryogenesis, in both selfs and hybrids, identified deleterious changes in the fertilised hybrid seed as early as 2–4 days after pollination in some crosses. These observations suggest that the appropriate time to rescue chickpea × C. bijugum hybrids is at the early globular stage of embryogenesis (2–7 days old), which requires the development of a complex tissue culture medium. In contrast hybrids between chickpea × C. pinnatifidum abort later (up to 15–20 days old) at the heart-shaped or torpedo stages, and are easier to rescue in vitro. Genotype also plays a significant role in the ability of immature selfed ovules to germinate in vitro. In this paper we report on the optimisation of␣protocols for rescueing immature embryos using selfed chickpea and its wild relatives in ovule, and subsequently to regenerate plantlets.
Keywordsgrain legume improvement interspecific hybridisation tissue culture wild relatives
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- Ahmad F, Slinkard AE (2003) Limitations to bridge-species facilitated alien gene transfers in chickpea: pre-fertilization events J. Genet. Breed. 57: 69–74Google Scholar
- Geerts P, Mergeai G, Baudoin JP (1999) Rescue of heart-shaped embryos and plant regeneration of Phaseolus polyanthus Greenm. and Phaseolus vulgaris L. Biotechnol Agron. Soc. Environ. 3: 141–148Google Scholar
- Geerts P, Toussaint A, Mergeai G, Baudoin JP (2002) Study of early abortion in reciprocal crosses between Phaseolus vulgaris L. and Phaseolus polyanthus Greenm Biotechnol. Agron. Soc. Environ. 6: 109–119Google Scholar
- Knights EJ, Brinsmead RB, Fordyce M, Wood J, Kelly AG, Harden S (2002) Use of wild relative Cicer echinospermum in chickpea improvement In: Mc Comb JA (eds) ‘Plant Breeding for the 3rd Millenium’, 12th Australasian Plant Breeding Conference. Australasian Plant Breeding Association Inc., Perth Western Australia, pp 150–154Google Scholar
- Malhotra RS, Singh KB, Di Vito M, Greco N, Saxena MC (2002) Registration of ILC 10765 and ILC 10766 chickpea germplasm lines resistant to cyst nematode Crop Sci. 42: 1756Google Scholar
- Mallikarjuna N (2003) Wide hybridisation in important food legumes. In: Jaiwal PK, Singh RP (eds) Improvement strategies of leguminosae biotechnology. Kluwer, Dordrecht, pp 155–170Google Scholar
- Singh KB, Di Vito M, Greco N, Saxena MC (1996) Registration of ILWC 292, a chickpea cyst nematode-resistant germplasm of Cicer reticulatum Ladiz Crop Sci. 36: 1421–1422Google Scholar
- Singh NP, Singh A, Asthana AN (1999) Studies on inter-specific crossability barriers in chickpea Ind. J. Pulses Res. 12: 13–19Google Scholar
- Stamigna C, Crino P, Saccardo F (2000) Wild relatives of chickpea: multiple disease resistance and problems to introgression in the cultigen J. Genet. Breed. 54: 213–219Google Scholar
- Swamy AVSR, Khanna VK (1991) Pollen grain germination and pollen tube growth following interspecific pollinations in Cicer Sabrao Journal 23: 137–145Google Scholar