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The influence of FeEDDHA in red raspberry cultures during shoot multiplication and adventitious regeneration from leaf explants

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Abstract

We examined the effect of FeEDDHA on multiplication via axillary branching and adventitious shoot regeneration from leaf explants in five red raspberry cultivars. When applied during multiplication, FeEDDHA reduced chlorosis, increased content of chlorophyll and iron but had no effect on the number of side shoots in four of the five cultivars. Addition of FeEDDHA to regeneration medium increased the percentage of regenerating leaves in some cultivars and the number of adventitious shoots in all five cultivars.

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Abbreviations

BA:

6-benzylaminopurine

BMM:

basic multiplication medium

BRM:

basic regeneration medium

FeEDTA:

ethylenediaminetetraacetate ferric sodium

FeEDDHA:

ethylenediamine di- 2-hydroxy-phenyl acetate ferric

IBA:

indole-3-butyric acid

MS:

Murashige and Skoog medium

TDZ (=thidiazuron):

N-1,2,3-(thiadiazol-5-yl)urea

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Acknowledgement

We thank Mrs L. Ogórek for technical assistance.

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Authors and Affiliations

  1. Research Institute of Pomology and Floriculture, Pomologiczna 18, 96-100 , Skierniewice, Poland

    Marta Zawadzka & Teresa Orlikowska

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  1. Marta Zawadzka
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  2. Teresa Orlikowska
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Correspondence to Teresa Orlikowska.

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Zawadzka, M., Orlikowska, T. The influence of FeEDDHA in red raspberry cultures during shoot multiplication and adventitious regeneration from leaf explants. Plant Cell Tiss Organ Cult 85, 145–149 (2006). https://doi.org/10.1007/s11240-005-9063-1

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  • DOI: https://doi.org/10.1007/s11240-005-9063-1

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