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In vitro plant regeneration of Arachis correntina (Leguminosae) through somatic embryogenesis and organogenesis

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Abstract

In vitro protocols for plant regeneration of Arachis correntina through both somatic embryogenesis and organogenesis were developed using immature leaves as explants. Morphologically normal somatic embryos were obtained on culture media composed of 20.70 or 41.41 μM picloram (PIC) with the addition of 0.044 μM 6-benzylaminopurine (BA), resulting in a 33 and 24% of conversion into plants, respectively. The source of explants and the developmental stage of the leaves had a marked effect on somatic embryogenesis. The second folded immature leaves from in vitro growing plants were the most responsive producing up to 30% embryogenesis in MS+41.41 μM PIC. Embryos converted into plants after transfer to MS medium devoid of growth regulators and these plants were successfully acclimatised. Adventitious shoots were obtained on culture media supplemented with 2,4-dichlorophenoxyacetic acid (2,4-D) or naphthaleneacetic acid (NAA) with or without 0.044 μM BA, achieving plant regeneration in the induction media. The highest percentage of bud formation was obtained on culture medium composed of␣MS+10.74 μM NAA+0.044 μM BA (12.5%). Roots were formed on all culture media tested. Regenerated plants were transferred to pots and grew well under greenhouse conditions.

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Abbreviations

BA:

6-benzylaminopurine

2,4-D:

2,4-dichlorophenoxyacetic acid

NAA:

naphthaleneacetic acid

PIC:

picloram-4-amino-3,5,6-trichloropicolinic acid

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Acknowledgements

HY Rey and LA Mroginski are members of the␣National Research Council (CONICET) of Argentina. We gratefully acknowledge the financial support received from UNNE, CABBIO, CONICET, and USDA.

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Authors and Affiliations

  1. Facultad de Ciencias Agrarias (UNNE), Instituto de Botánica del Nordeste (IBONE), Sargento Cabral 2131, 3400, Corrientes, Argentina

    María Laura Vidoz, Pablo Klusacek, Hebe Yolanda Rey & Luis Amado Mroginski

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  1. María Laura Vidoz
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  2. Pablo Klusacek
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  3. Hebe Yolanda Rey
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  4. Luis Amado Mroginski
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Correspondence to María Laura Vidoz.

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Vidoz, M.L., Klusacek, P., Rey, H.Y. et al. In vitro plant regeneration of Arachis correntina (Leguminosae) through somatic embryogenesis and organogenesis. Plant Cell Tiss Organ Cult 86, 111–115 (2006). https://doi.org/10.1007/s11240-005-9062-2

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  • DOI: https://doi.org/10.1007/s11240-005-9062-2

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