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Agrobacterium-mediated transient transformation of marigold (Tagetes erecta)

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Hypocotyls, roots, leaf sections and shoot tips from Tagetes erecta plantlets were inoculated with Agrobacterium tumefaciens, harboring the binary vector pCAMBIA2301, containing the β-glucuronidase gene. Histochemical GUS assays of infected tissues showed transient gus gene expression after 3 days.

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  • Delgado-Vargas F, Jiménez AR, Paredes-López O, (2000) Natural pigments: carotenoids, anthocyanins, and betalains – characteristics, biosíntesis, processing and stability Crit. Rev. Food Sci. Nutr. 40:173–289

    Article  PubMed  CAS  Google Scholar 

  • Dubey VS, Bhala R, Luthra R, (2003) An overview of the non-mevalonate pathway for terpenoid biosynthesis in plants J. Biosci. 28:637–646

    Article  PubMed  CAS  Google Scholar 

  • Giri A, Narasu ML, (2000) Transgenic hairy roots: recent trends and applications Biotechnol. Adv. 18:1–22

    Article  PubMed  CAS  Google Scholar 

  • Humara JM, Lopez M, Ordas RJ, (1993) Agrobacterium tumefaciens-mediated transformation of Pinus pinea L. cotyledons: an assessment of factors influencing the efficiency of uidA gen transfer Plant Cell Rep. 19:51–58

    Article  Google Scholar 

  • Jefferson R, (1987) Assaying chimeric genes in plants: the GUS gene fusion system Plant Mol. Biol. Rep. 5:387–405

    Article  CAS  Google Scholar 

  • Misra P, Datta SK, (1999) In vitro propagation of white marigold (Tagetes erecta L.) through shoot tip proliferation Curr. Sci. 77:1138–1140

    Google Scholar 

  • Robert ML, Herrera JL, Contreras F, Scorer KN, (1987) In vitro propagation of Agave fourcroydes Lem. (Henequen) Plant Cell Tiss. Org. Cult. 8:37–48

    Article  CAS  Google Scholar 

  • Sambrook J, Fritsch EF, Maniatis T, (1989) Molecular cloning: A Laboratory Manual 2 edn Cold Spring Harbor Laboratory Press Cold Spring Harbor, New York

    Google Scholar 

  • Vanegas PE, Cruz-Hernández A, Valverde ME, Paredes-López O, (2002) Plant regeneration via organogenesis in marigold Plant Cell Tiss. Org. Cult. 69:279–283

    Article  CAS  Google Scholar 

  • Vasudevan P, Kashyap S, Sharma S, (1997) Tagetes: a multipurpose plant Bioresource Technol. 62:29–35

    Article  CAS  Google Scholar 

  • Zhang HX, Zeevaart JA, (1999) An efficient Agrobacterium tumefaciens-mediated transformation and regeneration system for cotyledons of spinach (Spinacia oleracea L.) Plant Cell Rep. 18:640–645

    Article  CAS  Google Scholar 

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This study was supported by the Consejo Nacional de Ciencia y Tecnología (CONACYT) from México (Grant No. 28643-B).

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Correspondence to Gregorio Godoy-Hernández.

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Godoy-Hernández, G., Berzunza, E.A., Concha, L.C. et al. Agrobacterium-mediated transient transformation of marigold (Tagetes erecta). Plant Cell Tiss Organ Cult 84, 365–368 (2006).

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