Skip to main content
Log in

Particle bombardment, a method for gene transfer in marigold

  • Research note
  • Published:
Plant Cell, Tissue and Organ Culture Aims and scope Submit manuscript


Marigold (Tagetes erecta) leaf explants were bombarded with plasmid pBI426 in a low pressure particle inflow gun. Transient expression assays were performed with different pressures and distances. The best transient expression (GUS) level was obtained with explants induced in regeneration medium and bombarded at 10.5 cm and 551.6 kPa. Stable expression assays were performed and the callus tissue was selected on regeneration medium supplemented with 100 mg l−1 kanamycin. Stable genetic transformation of the resistant plantlets was demonstrated by PCR and Southern blot analysis; more than one transgene copy was observed in the transformed plantlet genomes. This method can be used for marigold gene transfer.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Similar content being viewed by others


  • Christou P, (1996) Particle Bombardment for Genetic Engineering of Plants. Biotechnology Intelligence Unit Academic Press UK

    Google Scholar 

  • Cruz-Hernández A, Witjaksono, Litz RE, Gomez Lim M, (1998) Agrobacterium tumefaciens – mediated transformation of embryogenic avocado cultures and regeneration of somatic embryos Plant Cell Rep. 17: 497–503

    Article  Google Scholar 

  • Datla RSS, Hammerlindl JK, Pelcher LE, Crosby WL, Selvaraj G, (1991) A bifunctional fusion between β-glucuronidase and neomycin phosphotransferase: a broad-spectrum marker enzyme for plants Gene 101:239–246

    Article  PubMed  CAS  Google Scholar 

  • Davies KM, Bloor SJ, Spiller GB, Deroles SC, (1998) Production of yellow colour in flowers: redirection of flavonoid biosynthesis in Petunia Plant J. 13:259–266

    Article  CAS  Google Scholar 

  • Delgado-Vargas F, Jiménez AR, Paredes-López O, (2000) Natural pigments: carotenoids, anthocyanins and betalains. Characteristics, biosynthesis, processing and stability Crit. Rev. Food Sci. Nutr. 40:173–289

    Article  PubMed  CAS  Google Scholar 

  • Delgado-Vargas F, Paredes-López O, (1996) Correlation of HPLC and AOAC methods to assess the all-trans-lutein content in marigold flowers J. Sci. Food Agric. 72:283–290

    Article  CAS  Google Scholar 

  • Doyle JJ, Doyle JL, (1990) Isolation of plant DNA from fresh tissue Focus 12:12–14

    Google Scholar 

  • Finer JJ, Vain P, Jones MW, McMullen MD, (1992) Development of the particle inflow gun for DNA delivery to plant cells Plant Cell Rep. 11:323–328

    Article  CAS  Google Scholar 

  • Guzmán-Maldonado SH, Paredes-López O, (1999) Biotechnology for the improvement of nutritional quality of crop plants In: Paredes-López O, (ed) Molecular Biotechnology for Plant Food Production CRC Press Boca Raton pp 553–620

    Google Scholar 

  • Henken H, (1992) Chemical and physiological behavior of feed carotenoids and their effect on pigmentation Poultry Sci. 71:711–717

    Google Scholar 

  • Hunold R, Bronner R, Hahne G, (1994) Early events in microprojectile bombardment: cell viability and particle location Plant J. 5:593–604

    Article  CAS  Google Scholar 

  • Hunold R, Burrus M, Bronner R, Duret JP, Hahne G, (1995) Transient gene expression in sunflower (Helianthus annus L) following microprojectile bombardment Plant Sci. 105:95–109

    Article  CAS  Google Scholar 

  • James C, (2002) Global Review of Commercialized Transgenic Crops: 2002. International Service for the Acquisition of Agri-Biotech Applications Cornell University Ithaca, New York, USA 7.

    Google Scholar 

  • Jefferson RA, (1987) Assaying chimeric genes in plants: the GUS fusion system Plant Mol Biol Rep. 5:387–405

    Article  CAS  Google Scholar 

  • Medina AL, BeMiller JN, (1993) Marigold flower meal as a source of an emulsifying gum In: Janick J, Simon JE, (eds) New Crops Wiley New York pp 389–393

    Google Scholar 

  • Murashige T, Skoog F, (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures Physiol. Plant 15:472–497

    Article  Google Scholar 

  • Suzuki S, Supaibulwatana K, Mii M, Nakano M, (2001) Production of transgenic plants of the Lilliaceous ornamental plant Agapanthys praecox ssp. orientalis (Leighton) via Agrobacterium-mediated transformation of embryogenic calli. Plant Sci. 161:89–97

    Article  CAS  Google Scholar 

  • Tanaka Y, Tsuda S, Kusumi T, (1998) Metabolic engineering to modify flower color Plant Cell Physiol. 39:1119–1126

    CAS  Google Scholar 

  • Vanegas PE, Cruz-Hernández A, Valverde ME, Paredes-López O, (2002) Plant regeneration via organogenesis in marigold Plant Cell Tiss. Org. Cult. 69:279–283

    Article  CAS  Google Scholar 

  • Vasudevan P, Kashyap S, Sharma S, (1997) Tagetes: a multipurpose plant Bioresource Technol. 62:29–33

    Article  CAS  Google Scholar 

  • Yepes LM, Mittak V, Pang SZ, Gonsalves C, Slightom JL, Gonsalves D, (1995) Biolistic transformation of chrysanthemum with the nucleocapsid gene of tomato spotted wilt virus Plant Cell Rep. 14:694–698

    Article  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations


Corresponding author

Correspondence to O. Paredes-López.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Vanegas, P., Valdez-Morales, M., Valverde, M. et al. Particle bombardment, a method for gene transfer in marigold. Plant Cell Tiss Organ Cult 84, 359–363 (2006).

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: