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Particle bombardment, a method for gene transfer in marigold

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Abstract

Marigold (Tagetes erecta) leaf explants were bombarded with plasmid pBI426 in a low pressure particle inflow gun. Transient expression assays were performed with different pressures and distances. The best transient expression (GUS) level was obtained with explants induced in regeneration medium and bombarded at 10.5 cm and 551.6 kPa. Stable expression assays were performed and the callus tissue was selected on regeneration medium supplemented with 100 mg l−1 kanamycin. Stable genetic transformation of the resistant plantlets was demonstrated by PCR and Southern blot analysis; more than one transgene copy was observed in the transformed plantlet genomes. This method can be used for marigold gene transfer.

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Correspondence to O. Paredes-López.

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Vanegas, P., Valdez-Morales, M., Valverde, M. et al. Particle bombardment, a method for gene transfer in marigold. Plant Cell Tiss Organ Cult 84, 359–363 (2006). https://doi.org/10.1007/s11240-005-9030-x

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  • DOI: https://doi.org/10.1007/s11240-005-9030-x

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