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A modeling approach of the in vitro conversion of oil palm (Elaeis guineensis) somatic embryos

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Abstract

A mathematical model for the growth and conversion of somatic embryos was developed with the aim of monitoring the large scale production of oil palm microplants. The predicted biomass of somatic embryos obtained and subcultured (B n ), together with the number of harvested shoots (Sh n ) – two key parameters for production forecasts – have been modeled for seven different shoot harvesting procedures. For the four different clonal lines studied, observed differences between experimental B n values at the end of each culture cycle and their theoretical counterpart generated by mathematical models were found to range between −30% to +14% at the end of the first 6-weeks culture cycle, then from −50% to +70% after the 6th subculturing operation (36 weeks). Concerning the predicted number of shoots harvested after conversion of somatic embryos (Sh n ), average variations between experimental and theoretical values ranged between −45% and +41%. Predicted values for biomass (B n ) between two culture cycles were found to vary slightly (+6% to +10%) indicating that the production of embryo biomass, as predicted by the model, was rather stable, for a given clonal line, from one 6-week cycle to another. The established model could thus be regarded as valid and the variations observed for B n and Sh n were found to be acceptable when compared to the those described by other models. Taken as a whole, predicted values for the two studied production parameters were in agreement with the corresponding experimental data (correlation=0.98).

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Acknowledgements

Dr Alian Rival's work was supported through a Marie Curie Outgoing International Fellowship from the 6th Framework Program of the European Commission.

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Author notes

  1. Alain Rival

    Present address: CSIRO Plant Industry, GPO Box 1600, Canberra, 2601, ACT, Australia

Authors and Affiliations

  1. Programme Palmier, Laboratoire de culture in vitro, CNRA Station de La Mé13, BP 989, Abidjan 13, Côte d’Ivoire

    Eugéne E. Konan

  2. UPR28, Cirad Cultures Pérennes, TA 80/03, Montpellier Cedex 5, 34398, France

    Tristan Durand-Gasselin & Albert Flori

  3. Laboratoire de Physiologie Végétale, UFR Sciences de la Nature, Université Abobo-Adjamé, Abidjan 02, 02 BP 801, Côte d’Ivoire

    Justin Y. Kouadio

  4. UMR 1098, BP 64501, 34394, Montpellier Cedex 5, France

    Alain Rival

Authors
  1. Eugéne E. Konan
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  2. Tristan Durand-Gasselin
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  3. Justin Y. Kouadio
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  4. Albert Flori
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  5. Alain Rival
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Correspondence to Alain Rival.

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Konan, E.E., Durand-Gasselin, T., Kouadio, J.Y. et al. A modeling approach of the in vitro conversion of oil palm (Elaeis guineensis) somatic embryos. Plant Cell Tiss Organ Cult 84, 100–113 (2006). https://doi.org/10.1007/s11240-005-9010-1

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  • DOI: https://doi.org/10.1007/s11240-005-9010-1

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