Fertile somatic hybrids were obtained via symmetric electrofusion of protoplasts from two combinations of tetraploid cotton (G. hirsutum cv. Coker 201, AD genome) and diploid wild cottons G. bickii (G genome) and G. stockii (E genome), respectively. Observation by morphological, flow cytometric analysis, chromosome counting and RAPD analysis of the tested hybrids of Coker 201 + G. bickii and Coker 201 + G. stockii confirmed the regenerated plants as hybrid status. Cytological investigation of the metaphase root-tip cells revealed there were 78 chromosomes in the hybrids. Flow cytometric analysis showed the tested plants had a relative DNA contents close to the total DNA contents of the two parents. RAPD analysis revealed the hybrids contained specific genomic fragments from both fusion partners, further confirmed their hybridity. The morphology of the hybrids was intermediate between the two fusion partners. The hybrid plants were successfully transferred to the soil, and they bloomed and set bolls. It is sure that the new hexaploids developed by cell fusion would contribute to cotton breeding through backcrossing with the elite genotypes of G. hirsutum.
This is a preview of subscription content, log in to check access.
Buy single article
Instant access to the full article PDF.
Price includes VAT for USA
Subscribe to journal
Immediate online access to all issues from 2019. Subscription will auto renew annually.
This is the net price. Taxes to be calculated in checkout.
II Atanassov SA Atanassova AI Dragoeva AI Atanassova (1998) ArticleTitleA new CMS source in Nicotiana developed via somatic cybridization between N. tabacum and N. alata Theor. Appl. Genet. 97 982–985 Occurrence Handle10.1007/s001220050980
PC Binsfeld R Wingender H Schnabl (2000) ArticleTitleCharacterization and molecular analysis of transgenic plants obtained by microprotoplast fusion in sunflower Theor. Appl. Genet. 101 1250–1258 Occurrence Handle10.1007/s001220051604
C Bushra Y Afshan H Tayyab S Riazuddin (1999) ArticleTitleMini-scale genomic DNA extraction from cotton Plant Mol. Bio. Rep. 17 1–7 Occurrence Handle10.1023/A:1017213630972
T Cardi ED Earle (1997) ArticleTitleProduction of new CMS Brassica oleracea by transfer of ‘Anand’ cytoplasm from B. rapa through protoplast fusion Theor. Appl. Genet. 94 204–212 Occurrence Handle10.1007/s001220050401
ED Earle M Temple TW Walters (1992) ArticleTitleOrganelle assortment and mitochondrial DNA rearrangements in Brassica somatic hybrids and cybrids Physiol. Plant. 85 325–333 Occurrence Handle10.1034/j.1399-3054.1992.850228.x
PA Fryxell (1992) ArticleTitleA revised taxonomic interpretation of Gossypium L. (Malvaceae) Rhoedea 2 IssueID2 108–165
OL Gamborg RA Miller K Ojima (1968) ArticleTitleNutrient requirements of suspension cultures of soybean root cells Exp. Cell Res. 50 151–158 Occurrence Handle10.1016/0014-4827(68)90403-5 Occurrence Handle5650857
HJ Henn R Wingender H Schnabl (1998) ArticleTitleRegeneration of␣fertile interspecific hybrids from cell fusion between Helianthus annuus L. and wild Helianthus species Plant Cell Rep. 18 220–224 Occurrence Handle10.1007/s002990050560
R Kumria VG Sunnichan DK Das SK Gupta VS Reddy RK Bhatnagar S Leelavathi (2003) ArticleTitleHigh-frequency somatic embryo production and maturation into normal plants in cotton (Gossypium hirsutum) through metabolic stress Plant Cell Rep. 21 635–639 Occurrence Handle12789412
ZL Liang (1999a) Genetics and Breeding of Distant Hybridization in Cotton Science Press Beijing 1–2
ZL Liang (1999b) Genetics and Breeding of Distant Hybridization in Cotton Science Press Beijing 202–208
M Miranda F Ikeda T Endo T Moriguchi M Omura (1997) ArticleTitleChromosome markers and alterations in mitotic cells from interspecific Citrus somatic hybrids analysed by fluorochrome staining Plant Cell Rep. 16 807–812 Occurrence Handle10.1007/s002990050325
T Murashige F Skoog (1962) ArticleTitleA revised media for rapid growth and bioassays with tobacco tissue culture Physiol. Plant. 15 473–479
KS Ramulu P Dijkhuis E Rutgers J Blass FA Krens WHJ Verbeek CM Colijn-Hooymans HA Verhoeven (1996) ArticleTitleIntergeneric transfer of a partial genome and direct production of monosomic addition plants by microprotoplast fusion Theor. Appl. Genet. 92 316–325 Occurrence Handle10.1007/s001220050130
Y Sun X Zhang Y Nie X Guo S Jin S Liang (2004) ArticleTitleProduction and characterization of somatic hybrids between upland cotton (Gossypium hirsutum) and wild cotton (G.␣klotzschianum Anderss) via electrofusion Theor. Appl. Genet. 109 472–479 Occurrence Handle10.1007/s00122-004-1663-3 Occurrence Handle15114473
J Wu X Zhang Y Nie S Jin S Liang (2004) ArticleTitleFactors affecting somatic embryogenesis and plant regeneration from a range of recalcitrant genotypes of Chinese cottons (Gossypium hirsutum L.) In Vitro Cell. Dev. Biol. – Plant 40 371–375 Occurrence Handle10.1079/IVP2004535
About this article
Cite this article
Sun, Y., Zhang, X., Nie, Y. et al. Production of fertile somatic hybrids of Gossypium hirsutum + G. bickii and G. hirsutum + G. stockii via protoplast fusion. Plant Cell Tiss Organ Cult 83, 303–310 (2005). https://doi.org/10.1007/s11240-005-7770-2
- Gossypium hirsutum
- G. bickii
- G. stockii
- protoplast fusion
- somatic hybrid